短杆菌属R312广谱酰胺酶的克隆及一级结构：与铜绿假单胞菌amiE产物高度同源。
Cloning and primary structure of the wide-spectrum amidase from Brevibacterium sp. R312: high homology to the amiE product from Pseudomonas aeruginosa.
作者信息
Soubrier F, Lévy-Schil S, Mayaux J F, Pétré D, Arnaud A, Crouzet J
机构信息
Département Biotechnologie, Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, Vitry sur Seine, France.
出版信息
Gene. 1992 Jul 1;116(1):99-104. doi: 10.1016/0378-1119(92)90635-3.
A Brevibacterium sp. R312 DNA fragment encoding the wide-spectrum amidase (EC 3.5.1.4) has been cloned and sequenced, using limited amino acid (aa) sequence information obtained from the purified enzyme. The deduced aa sequence showed more than 80% strict identity with the Pseudomonas aeruginosa aliphatic amidase, the product of the amiE gene, suggesting a horizontal transfer of the gene during evolution between Gram+ and Gram- bacteria.
利用从纯化酶中获得的有限氨基酸序列信息，已克隆并测序了编码广谱酰胺酶（EC 3.5.1.4）的短短芽孢杆菌属R312 DNA片段。推导的氨基酸序列与铜绿假单胞菌脂肪族酰胺酶（amiE基因产物）显示出超过80%的严格一致性，这表明该基因在革兰氏阳性菌和革兰氏阴性菌的进化过程中发生了水平转移。
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