Haase A, Smith-Vaughan H, Melder A, Wood Y, Janmaat A, Gilfedder J, Kemp D, Currie B
Menzies School of Health Research, Casuarina, Northern Territory, Australia.
J Clin Microbiol. 1995 Jul;33(7):1687-90. doi: 10.1128/jcm.33.7.1687-1690.1995.
Ribotyping has previously been used for epidemiological studies of Burkholderia pseudomallei (previously Pseudomonas pseudomallei). We show here that random amplified polymorphic DNA (RAPD) analysis allows subdivision of strains of the same ribotype. With five different primers, no two epidemiologically unrelated isolates of any single ribotype in this study of 102 isolates from humans, goats, cats, and soil had identical RAPD patterns. Conversely, RAPD analysis showed clonality for isolates from each of two animal outbreaks of melioidosis and from a nontropical focus of animal and human melioidosis spanning 25 years. Some soil isolates were identical to epidemiologically related animal and human isolates as determined by RAPD typing. There was no evidence that the clinical outcome of melioidosis was related to RAPD patterns.
核糖体分型先前已用于类鼻疽伯克霍尔德菌（以前称为类鼻疽假单胞菌）的流行病学研究。我们在此表明，随机扩增多态性DNA（RAPD）分析可对相同核糖体分型的菌株进行细分。在这项对来自人类、山羊、猫和土壤的102株分离株的研究中，使用五种不同的引物，任何单一核糖体分型的两株流行病学无关的分离株都没有相同的RAPD模式。相反，RAPD分析显示，两次动物类鼻疽病暴发以及一个跨越25年的动物和人类类鼻疽病非热带疫源地的各分离株均具有克隆性。通过RAPD分型确定，一些土壤分离株与流行病学相关的动物和人类分离株相同。没有证据表明类鼻疽病的临床结果与RAPD模式有关。
