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低氧诱导因子-1α(ODD)与hARD1之间的相互作用不会诱导低氧诱导因子-1α的乙酰化和稳定性破坏。

Interaction between HIF-1 alpha (ODD) and hARD1 does not induce acetylation and destabilization of HIF-1 alpha.

作者信息

Arnesen Thomas, Kong Xianguo, Evjenth Rune, Gromyko Darina, Varhaug Jan Erik, Lin Zhao, Sang Nianli, Caro Jaime, Lillehaug Johan R

机构信息

Department of Molecular Biology, University of Bergen, N-5020 Bergen, Norway.

出版信息

FEBS Lett. 2005 Nov 21;579(28):6428-32. doi: 10.1016/j.febslet.2005.10.036. Epub 2005 Nov 2.

Abstract

Hypoxia inducible factor-1 alpha (HIF-1 alpha) is a central component of the cellular responses to hypoxia. Hypoxic conditions result in stabilization of HIF-1 alpha and formation of the transcriptionally active HIF-1 complex. It was suggested that mammalian ARD1 acetylates HIF-1 alpha and thereby enhances HIF-1 alpha ubiquitination and degradation. Furthermore, ARD1 was proposed to be down-regulated in hypoxia thus facilitating the stabilization of HIF-1 alpha. Here we demonstrate that the level of human ARD1 (hARD1) protein is not decreased in hypoxia. Moreover, hARD1 does not acetylate and destabilize HIF-1 alpha. However, we find that hARD1 specifically binds HIF-1 alpha, suggesting a putative, still unclear, connection between these proteins.

摘要

缺氧诱导因子-1α(HIF-1α)是细胞对缺氧反应的核心成分。缺氧条件导致HIF-1α稳定并形成具有转录活性的HIF-1复合物。有人提出哺乳动物ARD1使HIF-1α乙酰化,从而增强HIF-1α的泛素化和降解。此外,有人认为ARD1在缺氧时下调,从而促进HIF-1α的稳定。在此我们证明,缺氧时人ARD1(hARD1)蛋白水平并未降低。此外,hARD1不会使HIF-1α乙酰化并使其不稳定。然而,我们发现hARD1特异性结合HIF-1α,表明这些蛋白之间存在一种尚不清楚的假定联系。

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本文引用的文献

2
Tumor hypoxia and cancer progression.
Cancer Lett. 2006 Jun 8;237(1):10-21. doi: 10.1016/j.canlet.2005.05.028. Epub 2005 Jul 5.
4
Negative and positive regulation of HIF-1: a complex network.
Biochim Biophys Acta. 2005 Jul 25;1755(2):107-20. doi: 10.1016/j.bbcan.2005.05.001.
5
Analysis of ARD1 function in hypoxia response using retroviral RNA interference.
J Biol Chem. 2005 May 6;280(18):17749-57. doi: 10.1074/jbc.M412055200. Epub 2005 Mar 8.
6
Binding of natively unfolded HIF-1alpha ODD domain to p53.
Mol Cell. 2005 Jan 7;17(1):11-21. doi: 10.1016/j.molcel.2004.11.019.
7
Angiogenesis--a self-adapting principle in hypoxia.
EXS. 2005(94):163-80. doi: 10.1007/3-7643-7311-3_12.
8
The hypoxia-inducible factor and tumor progression along the angiogenic pathway.
Int Rev Cytol. 2005;242:157-213. doi: 10.1016/S0074-7696(04)42004-X.
9
The role of hypoxia-induced factors in tumor progression.
Oncologist. 2004;9 Suppl 5:10-7. doi: 10.1634/theoncologist.9-90005-10.
10
Hypoxia-inducible factors and hypoxic cell death in tumour physiology.
Ann Med. 2004;36(7):530-9. doi: 10.1080/07853890410018231.

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