一种用于检测和定量血红素过氧化物酶氯化活性的比色法。
A colorimetric method for detection and quantification of chlorinating activity of hemeperoxidases.
作者信息
Manoj Kelath Murali, Hager Lowell P
机构信息
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
出版信息
Anal Biochem. 2006 Jan 1;348(1):84-6. doi: 10.1016/j.ab.2005.10.014. Epub 2005 Oct 26.
A method of detecting and assaying the halogenating activity of hemeperoxidases using the colored substrate, thionin, is reported here. In the presence of suitable amounts of peroxide and chloride, chloroperoxidase chlorinates thionin and bleaches the intense color of this substrate. The kinetics was quite similar to that of the established monochlorodimedone assay. Therefore, the thionin assay can be taken as an index of chlorinating activity. This reaction affords an escape from background problems and allows easy processing of large volumes of samples.
本文报道了一种使用有色底物硫堇检测和测定血红素过氧化物酶卤化活性的方法。在适量的过氧化物和氯化物存在下，氯过氧化物酶使硫堇氯化并使该底物的强烈颜色褪去。其动力学与已确立的一氯二甲基酮测定法非常相似。因此，硫堇测定法可作为氯化活性的指标。该反应避免了背景问题，并且便于处理大量样品。
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