在无质子去耦情况下生物固体中通过高效自旋-自旋标量耦合介导的碳-13同核极化转移

Efficient spin-spin scalar coupling mediated C-13 homonuclear polarization transfer in biological solids without proton decoupling.

作者信息

Mou Yun, Chao John Chin Hao, Chan Jerry C C

机构信息

Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei, Taiwan.

出版信息

Solid State Nucl Magn Reson. 2006 Jun;29(4):278-82. doi: 10.1016/j.ssnmr.2005.10.004. Epub 2005 Nov 10.

DOI:10.1016/j.ssnmr.2005.10.004

PMID:16289520

Abstract

We demonstrate that an efficient C' <--> C alpha polarization transfer based on J-coupling can be realized under fast magic-angle spinning (MAS) condition without 1H decoupling. Experimental results are presented for model crystalline compounds as well as a non-crystalline 17-residue polypeptide MB(i + 4)EK. Measurements on MB(i + 4)EK demonstrate that 53% of the initial C' polarization was transferred to the cross peaks at 7.05 T under 25 kHz MAS spinning.

摘要

我们证明，基于J耦合的高效C'⇄Cα极化转移可以在快速魔角旋转（MAS）条件下实现，而无需进行1H去耦。给出了模型晶体化合物以及一种非晶体17残基多肽MB(i + 4)EK的实验结果。对MB(i + 4)EK的测量表明，在25 kHz MAS旋转下，在7.05 T时，53%的初始C'极化转移到了交叉峰上。