全氘代蛋白质的同核混合序列。
Homonuclear mixing sequences for perdeuterated proteins.
机构信息
Department of Chemistry MC3113, Columbia University, 3000 Broadway, New York, NY 10027, USA.
出版信息
J Magn Reson. 2011 Jan;208(1):122-7. doi: 10.1016/j.jmr.2010.10.015. Epub 2010 Oct 26.
Abstract
We tested the performance of several (13)C homonuclear mixing sequences on perdeuterated microcrystalline ubiquitin. All sequences were applied without (1)H decoupling and at relatively low MAS frequencies. We found that RFDR gave the highest overall transfer efficiency and that DREAM performs surprisingly well under these conditions being twice as efficient in the aliphatic region of the spectrum than the other mixing sequences tested.
摘要
我们测试了几种（13）C 同核混合序列在氘代微结晶泛素上的性能。所有序列均未应用（1）H 去耦且在相对较低的 MAS 频率下应用。我们发现 RFDR 提供了最高的整体转移效率，并且在这些条件下 DREAM 的表现非常出色，在谱的脂肪族区域的效率是其他测试的混合序列的两倍。
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