Urano Hiromi, Fukuzaki Satoshi
Industrial Technology Center of Okayama Prefecture, 5301 Haga, Okayama 701-1296, Japan.
J Colloid Interface Sci. 2002 Aug 15;252(2):284-9. doi: 10.1006/jcis.2002.8500.
Kinetics of desorption of irreversibly adsorbed bovine serum albumin (BSA) from alumina (Al2O3) particles at various surface coverages (theta) was studied in a plug-flow column by feeding 0.05 M NaOH solution. The desorption curve obtained by plotting the logarithm of the amount of residual BSA against elution time was analyzed by using an integrated model that describes two simultaneous first-order processes. At each of theta, except for an initial lag time, the curve of BSA desorption could be reduced to the sum of two independent and simultaneous first-order processes occurring at different rates. The desorption rate constant (k(f)) for a faster-desorbing BSA (BSA(f)) was influenced by theta, especially above 0.7. On the other hand, the rate constant (k(s)) for a slower-desorbing BSA (BSA(s)) was fairly constant irrespective of theta and approximately 25- to 100-fold lower than k(f). The amount of BSA(f) estimated was proportional to theta, whereas that of BSA(s) reached a plateau value corresponding to 12.8% of saturation amount at theta above 0.42.
通过在活塞流柱中加入0.05 M NaOH溶液，研究了在不同表面覆盖率（θ）下不可逆吸附在氧化铝（Al2O3）颗粒上的牛血清白蛋白（BSA）的解吸动力学。通过绘制残留BSA量的对数与洗脱时间的关系得到解吸曲线，并使用描述两个同时发生的一级过程的积分模型进行分析。在每个θ下，除了初始滞后时间外，BSA解吸曲线可简化为以不同速率发生的两个独立且同时的一级过程的总和。快速解吸的BSA（BSA(f)）的解吸速率常数（k(f)）受θ影响，特别是在θ高于0.7时。另一方面，慢速解吸的BSA（BSA(s)）的速率常数（k(s)）与θ无关，相当恒定，且比k(f)低约25至100倍。估计的BSA(f)量与θ成正比，而BSA(s)的量在θ高于0.42时达到对应于饱和量12.8%的平台值。
