Carreras Maria Cecilia, Clara Franco Maria, P Converso Daniela, Finocchieto Paola, Galli Soledad, José Poderoso Juan
Laboratory of Oxygen Metabolism, University Hospital and School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
Methods Enzymol. 2005;396:399-414. doi: 10.1016/S0076-6879(05)96034-5.
For many years, mitochondrial respiration was thought to follow an "all or nothing" paradigm supporting the notion that in the normal O2 concentration range, respiration is mainly controlled by tissue demands. However, nitric oxide produced by cytosol or mitochondrial nitric oxide synthases adapts respiration to different physiologic conditions and increases the mitochondrial production of O2 active species that contributes to NO clearance. Because mitochondrial NO utilization is sensitive to environmental or hormonal modulation, and because diffusible active species, like H2O2, are able to regulate genes related to proliferation, quiescence, and death, we surmised that the two mechanisms converge to elicit the different responses in cell physiology.
多年来，线粒体呼吸被认为遵循一种“全或无”的模式，这支持了在正常氧气浓度范围内，呼吸主要由组织需求控制的观点。然而，由胞质溶胶或线粒体一氧化氮合酶产生的一氧化氮使呼吸适应不同的生理条件，并增加有助于清除一氧化氮的线粒体活性氧的产生。由于线粒体对一氧化氮的利用对环境或激素调节敏感，并且由于像过氧化氢这样的可扩散活性物质能够调节与增殖、静止和死亡相关的基因，我们推测这两种机制共同作用，在细胞生理学中引发不同的反应。
