采用固相萃取和高效液相色谱法测定血清中的4-氨基吡啶。

Determination of 4-aminopyridine in serum by solid-phase extraction and high-performance liquid chromatography.

作者信息

van der Horst A, de Goede P N, van Diemen H A, Polman C H, Martens H J

机构信息

Department of Pharmacy, Free University Hospital, Amsterdam, Netherlands.

出版信息

J Chromatogr. 1992 Feb 7;574(1):166-9. doi: 10.1016/0378-4347(92)80114-6.

DOI:10.1016/0378-4347(92)80114-6

PMID:1629282

Abstract

An assay for the determination of 4-aminopyridine in serum has been developed using 3,4-diaminopyridine as internal standard and reversed-phase high-performance liquid chromatography with detection at 244 nm. A mobile phase of acetonitrile-methanol-ethanol-1% ammonium carbonate (75:10:10:5) provided excellent separation of both compounds. Samples were extracted on solid-phase columns. The linearity, precision, recovery and the limit of detection were all sufficient for the routine use of this assay in clinical studies of patients treated with 4-aminopyridine.

摘要

已开发出一种使用3,4-二氨基吡啶作为内标，采用反相高效液相色谱法在244 nm波长处进行检测来测定血清中4-氨基吡啶的分析方法。乙腈-甲醇-乙醇-1%碳酸铵（75:10:10:5）的流动相能使两种化合物实现极佳分离。样品在固相柱上进行萃取。该分析方法的线性、精密度、回收率和检测限均足以用于4-氨基吡啶治疗患者的临床研究中的常规检测。