Wax Adam, Pyhtila John W, Graf Robert N, Nines Ronald, Boone Charles W, Dasari Ramachandra R, Feld Michael S, Steele Vernon E, Stoner Gary D
Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA.
J Biomed Opt. 2005 Sep-Oct;10(5):051604. doi: 10.1117/1.2102767.
Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria established in a previous a/LCI study to prospectively grade neoplastic progression. We exploit the depth resolution of a/LCI to exclusively examine the basal layer of the epithelium, approximately 50 to 100 microm beneath the tissue surface, without the need for exogenous contrast agents, tissue sectioning, or fixation. The results of two studies are presented that compare the performance of two a/LCI modalities. Overall, the combined studies show 91% sensitivity and 97% specificity for detecting dysplasia, using histopathology as the standard. In addition, the studies enable the effects of dietary chemopreventive agents, difluoromethylornithine (DFMO) and curcumin, to be assessed by observing modulation in the incidence of neoplastic change. We demonstrate that a/LCI is highly effective for monitoring neoplastic change and can be applied to assessing the efficacy of chemopreventive agents in the rat esophagus.
角分辨低相干干涉测量法（a/LCI）用于获取定量的、深度分辨的细胞核形态测量结果。我们将大鼠食管上皮组织中细胞核的平均直径和纹理与先前a/LCI研究中建立的分级标准进行比较，以对肿瘤进展进行前瞻性分级。我们利用a/LCI的深度分辨率专门检查上皮的基底层，该层位于组织表面下方约50至100微米处，无需使用外源性造影剂、组织切片或固定。本文展示了两项研究的结果，比较了两种a/LCI模式的性能。总体而言，以组织病理学为标准，综合研究显示检测发育异常的灵敏度为91%，特异性为97%。此外，这些研究能够通过观察肿瘤变化发生率的调节来评估饮食化学预防剂二氟甲基鸟氨酸（DFMO）和姜黄素的效果。我们证明a/LCI在监测肿瘤变化方面非常有效，可用于评估大鼠食管中化学预防剂的疗效。
