基于在日本大型斗鸡和白来航鸡杂交后代中进行基因分型的微卫星标记构建的鸡连锁图谱。

A chicken linkage map based on microsatellite markers genotyped on a Japanese Large Game and White Leghorn cross.

作者信息

Takahashi H, Tsudzuki M, Sasaki O, Niikura J, Inoue-Murayama M, Minezawa M

机构信息

Genebank, National Institute of Agrobiological Sciences (NIAS), Tsukuba 305-8602, Japan.

出版信息

Anim Genet. 2005 Dec;36(6):463-7. doi: 10.1111/j.1365-2052.2005.01391.x.

DOI:10.1111/j.1365-2052.2005.01391.x

PMID:16293118

Abstract

A detailed linkage map is necessary for efficient detection of quantitative trait loci (QTL) in chicken resource populations. In this study, microsatellite markers isolated from a (CA)n-enriched library (designated as ABR Markers) were mapped using a population developed from a cross between Japanese Game and White Leghorn chickens. In total, 296 markers including 193 ABR, 43 MCW, 31 ADL, 22 LEI, 3 HUJ, 2 GCT, 1 UMA and 1 ROS were mapped by linkage to chicken chromosomes 1-14, 17-21, 23, 24, 26-28 and Z. In addition, five markers were assigned to the map based on the chicken draft genomic sequence, bringing the total number of markers on the map to 301. The resulting linkage map will contribute to QTL mapping in chicken.

摘要

详细的连锁图谱对于在鸡资源群体中高效检测数量性状基因座（QTL）至关重要。在本研究中，利用从日本斗鸡和白来航鸡杂交产生的群体，对从富含（CA）n的文库中分离出的微卫星标记（指定为ABR标记）进行了定位。总共通过与鸡的1-14号、17-21号、23号、24号、26-28号染色体以及Z染色体连锁，定位了296个标记，包括193个ABR标记、43个MCW标记、31个ADL标记、22个LEI标记、3个HUJ标记、2个GCT标记、1个UMA标记和1个ROS标记。此外，基于鸡的基因组草图序列将5个标记添加到图谱中，使图谱上标记的总数达到301个。所得的连锁图谱将有助于鸡的QTL定位。