Fukuhira Yukako, Kitazono Eiichi, Hayashi Takami, Kaneko Hiroaki, Tanaka Masaru, Shimomura Masatsugu, Sumi Yoshihiko
Department of Tissue Engineering Development, Innovation Research Institute, Teijin Ltd., 4-3-2, Asahigaoka, Hino, Tokyo 191-8512, Japan.
Biomaterials. 2006 Mar;27(9):1797-802. doi: 10.1016/j.biomaterials.2005.10.019. Epub 2005 Nov 15.
Honeycomb-patterned films have been reported to be useful for scaffolds of cell culture in tissue engineering. In the present study, we investigated a new compound, dioleoylphosphatidylethanolamine (DOPE), a naturally derived phospholipid having unsaturated fatty acid moieties, as a surfactant for fabricating honeycomb-patterned poly(d,l-lactide) (PLA) film. Only DOPE among commercially available phospholipids was useful as a surfactant, and it showed good solubility in PLA/chloroform solution and an excellent property for fabricating honeycomb-patterned film (the concentration of DOPE was from 0.2% to 20% by weight based on the weight of PLA). The pore size of the honeycomb was uniform, and all pores were interconnected with each other. The contact angle of water on the honeycomb-patterned film was affected by the amount of DOPE. Time-of-flight secondary ion mass spectrometer (TOF-SIMS) data suggested that DOPE was concentrated on the surface of the honeycomb-patterned film. To investigate cell proliferation and adhesion on the honeycomb-patterned film, NIH3T3 fibroblast cells were cultured on the film. The NIH3T3 cells adhered well on the honeycomb-patterned PLA film with DOPE (PLA-DOPE) and showed good cell proliferation compared to that on honeycomb-patterned PLA film fabricated with a copolymer (CAP) of dodecylacrylamide and omega-carboxyhexylacrylamide (PLA-CAP). These results suggest that the honeycomb-patterned PLA-DOPE can be applicable as a scaffold for cells with better profiles in comparison with PLA-CAP.
据报道，蜂窝状薄膜可用于组织工程中的细胞培养支架。在本研究中，我们研究了一种新的化合物——二油酰磷脂酰乙醇胺（DOPE），一种具有不饱和脂肪酸部分的天然衍生磷脂，作为制备蜂窝状聚（d，l-丙交酯）（PLA）薄膜的表面活性剂。在市售磷脂中，只有DOPE可用作表面活性剂，它在PLA/氯仿溶液中表现出良好的溶解性，并且具有制备蜂窝状薄膜的优异性能（DOPE的浓度基于PLA的重量为0.2%至20%重量）。蜂窝的孔径均匀，所有孔相互连通。蜂窝状薄膜上的水接触角受DOPE量的影响。飞行时间二次离子质谱仪（TOF-SIMS）数据表明，DOPE集中在蜂窝状薄膜的表面。为了研究细胞在蜂窝状薄膜上的增殖和粘附，将NIH3T3成纤维细胞培养在该薄膜上。与用十二烷基丙烯酰胺和ω-羧基己基丙烯酰胺的共聚物（CAP）制备的蜂窝状PLA薄膜（PLA-CAP）相比，NIH3T3细胞在含DOPE的蜂窝状PLA薄膜（PLA-DOPE）上粘附良好，并表现出良好的细胞增殖。这些结果表明，与PLA-CAP相比，蜂窝状PLA-DOPE可作为具有更好性能的细胞支架。
