Finger T E, Kanwal J S
Department of Cellular and Structural Biology, University of Colorado School of Medicine, Denver 80262.
J Comp Neurol. 1992 Jun 22;320(4):509-20. doi: 10.1002/cne.903200408.
The primary general visceral nucleus in goldfish (Carassius auratus) and catfish (Ictalurus punctatus) is located at the ventroposterior boundary of the vagal gustatory lobe and receives coelomic visceral, but not gustatory inputs. The neuronal tracer horseradish peroxidase (HRP) was employed to visualize sources of input to and ascending projections from the primary general visceral nucleus in these species. In addition, immunocytochemical techniques were utilized to define the cytological divisions within the pontine gustatory-visceral complex. The pontine secondary visceral nuclei in both catfish and goldfish contains numerous somata and fibers immunoreactive for calcitonin gene-related peptide (CGRP). In contrast, the secondary gustatory nuclei are devoid of fibers and cells immunoreactive for CGRP. In both the goldfish and the channel catfish, the primary general visceral nucleus receives input from the vagal gustatory lobe, as well as the medullary reticular formation. In the channel catfish, the primary general visceral nucleus projects bilaterally to the secondary visceral nucleus, which lies rostrolateral to the secondary gustatory nucleus in the dorsal pons. Fibers cross the midline via the rostral part of the isthmic commissure. Injection of HRP into the primary general visceral nucleus of a goldfish labels ascending fibers that project to a secondary visceral nucleus situated ventral, lateral, and rostral to the secondary gustatory complex. In general, the results indicate that general visceral systems ascend in parallel to gustatory systems within the brainstem, and that general visceral but not gustatory nuclei are immunoreactive for the peptide CGRP.
金鱼（Carassius auratus）和鲶鱼（Ictalurus punctatus）的初级一般内脏核位于迷走味觉叶的腹后边界，接收体腔内脏传入，但不接收味觉传入。使用神经元示踪剂辣根过氧化物酶（HRP）来显示这些物种初级一般内脏核的输入源和上行投射。此外，利用免疫细胞化学技术来界定脑桥味觉 - 内脏复合体中的细胞学分区。鲶鱼和金鱼的脑桥次级内脏核都含有大量对降钙素基因相关肽（CGRP）免疫反应阳性的细胞体和纤维。相比之下，次级味觉核没有对CGRP免疫反应阳性的纤维和细胞。在金鱼和沟鲶中，初级一般内脏核接收来自迷走味觉叶以及延髓网状结构的输入。在沟鲶中，初级一般内脏核双侧投射到位于脑桥背侧次级味觉核的 rostrolateral 的次级内脏核。纤维通过峡部连合的 Rostral 部分穿过中线。将HRP注入金鱼的初级一般内脏核会标记出上行纤维，这些纤维投射到位于次级味觉复合体腹侧、外侧和 Rostral 的次级内脏核。总体而言，结果表明一般内脏系统在脑干内与味觉系统平行上行，并且一般内脏核而非味觉核对肽CGRP免疫反应阳性。
