Liang Minglin, Wang Hongbo, Zhang Yuan, Lu Shi, Wang Zehua
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Cancer Biol Ther. 2006 Jan;5(1):28-33. doi: 10.4161/cbt.5.1.2234. Epub 2006 Jan 12.
To examine the expression of PDGF and its receptors in leiomyoma tissue and to investigate the regulation of PDGF on leiomyoma cell proliferation.
The expression of PDGF and its receptors was examined in 21 pairs of uterine leiomyoma and the adjacent myometrium tissues using an immunostaining method. Paired cultures of leiomyoma and normal myometrium cells were established and treated with PDGF. Total RNA was extracted from leiomyoma and myometrial cells for detection of the expression of proliferating cell nuclear antigen (PCNA) and collagen alpha1 (I, III) by semi-quantitative PCR.
The expression of PDGF-AA and PDGF-BB in leiomyoma tissue is obviously higher than that in the matched myometrial tissue (P < 0.05). In addition, the expression of PDGF-BB in secretory phase is higher than that in proliferative phase of the menstrual cycle (P = 0.027) in leiomyoma tissue. The expression of PCNA and collagen alpha1 (I) were increased in both leiomyoma and myometrial cells after treatment of PDGF, whereas the expression levels were greater in leiomyoma cells than that in myometrial cells.
The expression of PDGF and its receptors in leiomyoma and myometrial tissue varied during the menstrual cycle. PDGF may play a role in the pathogenesis of leiomyomas through a mechanism involved in not only the proliferation of leiomyoma cells but also excessive expression of extra cellular molecules.
检测血小板源性生长因子（PDGF）及其受体在平滑肌瘤组织中的表达，并探讨PDGF对平滑肌瘤细胞增殖的调控作用。
采用免疫染色法检测21对子宫平滑肌瘤及其相邻子宫肌层组织中PDGF及其受体的表达。建立平滑肌瘤细胞和正常子宫肌层细胞的配对培养体系，并给予PDGF处理。提取平滑肌瘤细胞和子宫肌层细胞的总RNA，采用半定量聚合酶链反应（PCR）检测增殖细胞核抗原（PCNA）和I、III型胶原α1链（collagen alpha1 (I, III)）的表达。
平滑肌瘤组织中PDGF-AA和PDGF-BB的表达明显高于配对的子宫肌层组织（P < 0.05）。此外，平滑肌瘤组织中分泌期PDGF-BB的表达高于增殖期（P = 0.027）。PDGF处理后，平滑肌瘤细胞和子宫肌层细胞中PCNA和I型胶原α1链的表达均增加，但平滑肌瘤细胞中的表达水平高于子宫肌层细胞。
PDGF及其受体在平滑肌瘤和子宫肌层组织中的表达在月经周期中存在差异。PDGF可能通过参与平滑肌瘤细胞增殖及细胞外分子过度表达的机制在平滑肌瘤发病中发挥作用。
