Shukla Jyoti, Rai Sunita, Singh V P
Department of Haematology and Blood Transfusion, Institute of Medical Sciences, Banaras Hindu University, Varanasi.
Indian J Pathol Microbiol. 2004 Apr;47(2):266-8.
Herein we are presenting the clinical, morphological and cytochemical characteristics of five cases of acute megakaryoblastic leukaemia (AML-M7) seen by us over a period of five years (Jan 1996-Dec 2000). Morphological assessment revealed marked polymorphism of blast cells and platelets both in the peripheral blood and bone marrow smears in all cases. Size of the blast cells ranged from very small to very large multinucleated cells, with variable chromatin pattern and number of nucleoli. More differentiated megakaryocytic cells showing cytoplasmic blebs, protrusions and platelet budding with bizarre platelet morphology were characteristic features suggesting the diagnosis. Cytochemical stains like myeloperoxidase, sudan black and PAS were positive in 5-15% of blast cell. Coagulation studies revealed a normal coagulation profile, whereas platelet studies showed marked impairment in aggregation of platelets with ADP and adrenalin with a normal PF-3 availability.
在此，我们呈现了在五年期间（1996年1月 - 2000年12月）我们所见到的5例急性巨核细胞白血病（AML - M7）的临床、形态学和细胞化学特征。形态学评估显示，在所有病例的外周血和骨髓涂片中，原始细胞和血小板均有明显的多态性。原始细胞大小从非常小到非常大的多核细胞不等，染色质模式和核仁数量各不相同。显示出细胞质小泡、突起以及伴有奇异血小板形态的血小板出芽的更分化的巨核细胞是提示诊断的特征性表现。髓过氧化物酶、苏丹黑和PAS等细胞化学染色在5% - 15%的原始细胞中呈阳性。凝血研究显示凝血谱正常，而血小板研究显示血小板对ADP和肾上腺素的聚集有明显损害，PF - 3活性正常。
