Paulous S, Emerman M, Keller R, Montagnier L, Cordonnier A
Unité d'Oncologie Virale (CNRS UA1157), Institut Pasteur, Paris, France.
J Gen Virol. 1992 Jul;73 ( Pt 7):1773-80. doi: 10.1099/0022-1317-73-7-1773.
The human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev stimulates expression of structural viral proteins via a target response element (RRE) located within gag-pol and env mRNAs. To analyse the HIV-2 Rev trans-activation effect on the expression of the envelope protein, we cloned a functionally active HIV-2 rev cDNA and showed that it contained four exons. Using transient expression assays, we mapped a 353 bp RRE fragment within the env gene of HIV-2 on which both HIV-1 and HIV-2 Rev could act. Interestingly, smaller fragments suppressed the use of additional splice sites within the env gene and caused envelope protein expression independent of Rev.
1型人类免疫缺陷病毒（HIV-1）调节蛋白Rev通过位于gag-pol和env mRNA内的靶反应元件（RRE）刺激病毒结构蛋白的表达。为了分析HIV-2 Rev对包膜蛋白表达的反式激活作用，我们克隆了一个功能活性的HIV-2 rev cDNA，并表明它包含四个外显子。通过瞬时表达分析，我们在HIV-2的env基因内定位了一个353 bp的RRE片段，HIV-1和HIV-2 Rev均可作用于该片段。有趣的是，较小的片段抑制了env基因内其他剪接位点的使用，并导致包膜蛋白的表达独立于Rev。
