Martín J, Albo C, Ortín J, Melero J A, Portela A
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Madrid, Spain.
J Gen Virol. 1992 Jul;73 ( Pt 7):1855-9. doi: 10.1099/0022-1317-73-7-1855.
A procedure to obtain RNA-free preparations containing the nucleoprotein (NP) and polymerase (P) proteins from influenza virus-infected cell extracts has been developed. The influenza virus P proteins present in these preparations copied small synthetic RNA molecules derived from plasmid sequences. In addition, RNA molecules encapsidated with the NP and P proteins were amplified and packaged into virus particles when transfected into influenza virus-infected cells. Thus, the preparations of NP and P proteins display features similar to those isolated from purified influenza virions, and represent an alternative for the preparation of active influenza virus P protein.
已开发出一种从感染流感病毒的细胞提取物中获取不含RNA的核蛋白（NP）和聚合酶（P）蛋白制剂的方法。这些制剂中存在的流感病毒P蛋白复制了源自质粒序列的小合成RNA分子。此外，当转染到感染流感病毒的细胞中时，被NP和P蛋白包裹的RNA分子会被扩增并包装成病毒颗粒。因此，NP和P蛋白制剂表现出与从纯化流感病毒粒子中分离出的制剂相似的特征，代表了一种制备活性流感病毒P蛋白的替代方法。
