Shen Wei, Li Yong, Huard Johnny
Growth and Development Laboratory of Children's Hospital of Pittsburgh, 4100 Rangos Research Center, Pittsburgh, PA 15213-2583, USA.
Infect Dis Clin North Am. 2005 Dec;19(4):1007-22. doi: 10.1016/j.idc.2005.07.005.
Tissue repair is a major issue in orthopedics. Many musculoskeletal tissues, including cartilage, meniscus, and the anterior cruciate ligament, heal poorly after injury. Recent studies have led to the identification of numerous growth factors and other gene products that can promote the regeneration of damaged musculoskeletal tissues. In the last century, the discovery and evolving use of antibiotics has significantly decreased the prevalence and severity of infectious diseases. In many orthopedic scenarios, however, treatment of infections can be difficult, and often involves a prolonged course of antibiotics with concomitant surgical interventions and loss of tissue. Although studies have demonstrated the successful transfer of target genes and the associated manipulation of the musculoskeletal tissue environment, researchers have made few attempts designed to use gene therapy to treat infectious musculoskeletal diseases in animal models. Before it is possible to use gene-based approaches to treat such diseases effectively, researchers must perform more studies to investigate the potential problems that may arise when using gene therapy in an infectious environment.
组织修复是骨科领域的一个重要问题。许多肌肉骨骼组织，包括软骨、半月板和前交叉韧带，受伤后愈合能力较差。最近的研究已鉴定出多种可促进受损肌肉骨骼组织再生的生长因子和其他基因产物。在上个世纪，抗生素的发现和不断发展的应用显著降低了传染病的发病率和严重程度。然而，在许多骨科情况下，感染的治疗可能很困难，通常需要长期使用抗生素，并伴有手术干预和组织缺失。尽管研究已证明在肌肉骨骼组织环境中成功实现了靶基因的转移及相关操作，但研究人员很少尝试在动物模型中使用基因疗法治疗感染性肌肉骨骼疾病。在能够有效利用基于基因的方法治疗此类疾病之前，研究人员必须进行更多研究，以调查在感染环境中使用基因疗法可能出现的潜在问题。
