Roper D Keith, Nakra Shamit
Department of Chemical Engineering, University of Utah, Salt Lake City, UT 84112, USA.
Anal Biochem. 2006 Jan 1;348(1):75-83. doi: 10.1016/j.ab.2005.10.019. Epub 2005 Nov 2.
Intrinsic adsorption rates of whole adenovirus type 5 (Ad5) onto a diethylaminoethyl (DEAE) anion exchange surface are measured for the first time by surface plasmon resonance (SPR). Fitting SPR sensorgrams to a two-compartment mass transport reaction model distinguishes intrinsic adsorption rates from slow diffusive Ad5 mass transport. Ad5 is a widely used viral vector for gene therapy that binds electrostatically to surfaces of cells and synthetics such as membranes, chromatographic resins, and glass. Increasing NaCl concentration from 4.8 to 14.4mM shifts binding of whole Ad5 from diffusion control to a regime where both sorption and diffusion affect binding. Intrinsic adsorption rates for Ad5-DEAE interaction are 16 times faster than intrinsic adsorption rates for Ad5 fiber knob interacting with soluble extracellular domain of coxsackievirus adenovirus receptors (s-CAR).
首次通过表面等离子体共振（SPR）测量了完整的5型腺病毒（Ad5）在二乙氨基乙基（DEAE）阴离子交换表面上的固有吸附速率。将SPR传感图拟合到双室质量传输反应模型中，可将固有吸附速率与缓慢扩散的Ad5质量传输区分开来。Ad5是一种广泛用于基因治疗的病毒载体，它通过静电作用与细胞表面以及诸如膜、色谱树脂和玻璃等合成材料结合。将NaCl浓度从4.8mM提高到14.4mM会使完整Ad5的结合从扩散控制转变为吸附和扩散都影响结合的状态。Ad5与DEAE相互作用的固有吸附速率比Ad5纤维结与柯萨奇病毒腺病毒受体（s-CAR）的可溶性细胞外结构域相互作用的固有吸附速率快16倍。
