Hughes S J, McShane P, Contractor H H, Gray D W R, Clark A, Johnson P R V
Islet Transplant Research Group, Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Transplant Proc. 2005 Oct;37(8):3444-5. doi: 10.1016/j.transproceed.2005.09.027.
Efficient islet isolation depends on the use of collagenase to digest the extracellular matrix within the islet-exocrine interface, the molecular structure of which is poorly understood. Recently it has been reported that transplantable yields of islets can be isolated from the tail segment of the pancreas alone. This study aimed to quantify and compare the amount of collagenase-resistant collagen VI within the islet-exocrine interface of the head, body, and tail of the human pancreas. Human adult pancreata (n = 5) were retrieved from heart-beating donors (age range, 40-62 years; cold ischemia times <10 hours). Tissue blocks from the head, body, and tail region of each pancreas were fixed in formalin and processed for immuno-labelling of collagen VI, which was quantified in the islet-exocrine interface using a Zeiss KS-400 image analysis system. Data were expressed as area of collagen at the interface relative to the islet area. Statistical analysis was done using paired t test. The mean islet areas in the head, body, and tail regions were not significantly different. Collagen VI was uniformly present within the islet-exocrine interface of all regions of the pancreas and was 0.326 +/- 0.064, 0.324 +/- 0.060, and 0.334 +/- 0.052 microm(2)/islet area (P = .441) in the head, body, and tail, respectively. The content of collagen VI within the islet-exocrine interface was uniform throughout all parts of the adult pancreas. Targeting this collagen subtype with novel collagenase blends may result in consistently improved islet yields and enable a wider number of available donor pancreata to be used.
高效的胰岛分离依赖于使用胶原酶来消化胰岛-外分泌腺界面处的细胞外基质，而其分子结构尚不清楚。最近有报道称，仅从胰腺尾部就能分离出可用于移植的胰岛产量。本研究旨在量化和比较人胰腺头部、体部和尾部的胰岛-外分泌腺界面中抗胶原酶的Ⅵ型胶原的含量。从心脏仍在跳动的供体（年龄范围40 - 62岁；冷缺血时间<10小时）获取5例成人胰腺。将每个胰腺头部、体部和尾部区域的组织块用福尔马林固定，并进行Ⅵ型胶原的免疫标记，使用蔡司KS - 400图像分析系统在胰岛-外分泌腺界面进行定量。数据表示为界面处胶原面积相对于胰岛面积。采用配对t检验进行统计分析。头部、体部和尾部区域的平均胰岛面积无显著差异。Ⅵ型胶原在胰腺所有区域的胰岛-外分泌腺界面均有均匀分布，在头部、体部和尾部的含量分别为0.326±0.064、0.324±0.060和0.334±0.052μm²/胰岛面积（P = 0.441）。在成人胰腺的所有部位，胰岛-外分泌腺界面内Ⅵ型胶原的含量是均匀的。用新型胶原酶混合物靶向这种胶原亚型可能会持续提高胰岛产量，并使更多可用的供体胰腺得以利用。
