血红素加氧酶-1转基因胰岛对移植的影响。

Effects of heme oxygenase-1 transgenic islets on transplantation.

作者信息

Tu C-F, Kuo C-H, Juang J-H

机构信息

Division of Biotechnology, Animal Technology Institute Taiwan, Miaoli, Taiwan.

出版信息

Transplant Proc. 2005 Oct;37(8):3463-7. doi: 10.1016/j.transproceed.2005.09.022.

DOI:10.1016/j.transproceed.2005.09.022

PMID:16298630

Abstract

Heme oxygenase-1 (HO-1) has been described as a protein capable of cytoprotection via radical scavenging and apoptosis prevention. The aim of this study was to analyze whether HO-1 overexpression in freshly isolated murine transgenic islets resulted in cell protection and improved in vivo functional performance after transplantation. We produced transgenic mice in which the human HO-1 transgene driven by chicken beta-actin promoter was expressed in the heart, liver, spleen, lung, kidney, muscle, intestine, and pancreas in Balb/c mice. One hundred fifty islets isolated from HO-1 transgenic and control Balb/c mice were syngeneically transplanted under the left kidney capsule of the streptozotocin-diabetic Balb/c mice. The recipients who underwent transplantation with HO-1 transgenic islets showed higher blood glucose than those with control islets at 4 weeks (320 +/- 25 vs 189 +/- 43 mg/dL; P < .05). Body weight was not significantly different between the 2 groups. Our data indicate transgenic islets with high HO-1 expression did not improve transplantation outcome.

摘要

血红素加氧酶-1（HO-1）被描述为一种能够通过清除自由基和预防细胞凋亡实现细胞保护的蛋白质。本研究旨在分析在新鲜分离的小鼠转基因胰岛中HO-1的过表达是否能在移植后带来细胞保护并改善体内功能表现。我们制备了转基因小鼠，其中由鸡β-肌动蛋白启动子驱动的人HO-1转基因在Balb/c小鼠的心脏、肝脏、脾脏、肺、肾脏、肌肉、肠道和胰腺中表达。从HO-1转基因小鼠和对照Balb/c小鼠分离出150个胰岛，同基因移植到链脲佐菌素诱导的糖尿病Balb/c小鼠的左肾包膜下。移植HO-1转基因胰岛的受体在4周时血糖水平高于移植对照胰岛的受体（320±25 vs 189±43 mg/dL；P<0.05）。两组间体重无显著差异。我们的数据表明，高表达HO-1的转基因胰岛并未改善移植结果。