Ferraresi Roberta, Troiano Leonarda, Roat Erika, Lugli Enrico, Nemes Elisa, Nasi Milena, Pinti Marcello, Fernandez Maria I Garcia, Cooper Edwin L, Cossarizza Andrea
University of Modena and Reggio Emilia School of Medicine, Department of Biomedical Sciences, Italy.
Free Radic Res. 2005 Nov;39(11):1249-58. doi: 10.1080/10715760500306935.
We have analyzed the anti- or pro-oxidant effects of the flavonoid quercetin (QU) by evaluating, in U937 cell line, hydrogen peroxide (H(2)O(2)), superoxide anion reduced glutathione (GSH) content, mitochondrial membrane potential, DNA content, phosphatidylserine exposure on the outer face of the plasma membrane and cell viability. Polychromatic flow cytometry was used to evaluate in the same cells several functional parameters. For short periods of treatment QU exerted an anti-oxidant effect (decrease in H(2)O(2) levels), whereas for long periods it showed a pro-oxidant activity (increase in ). In these conditions, GSH content was reduced, and this correlated with a lack of anti-oxidant activity of QU, which in turn could be correlated with proapoptotic activity of this molecule. Thus, QU can exert different effects (anti-/prooxidant) depending on exposure times and oxidative balance, and in particular on stores of GSH.
我们通过评估U937细胞系中的过氧化氢（H₂O₂）、超氧阴离子、还原型谷胱甘肽（GSH）含量、线粒体膜电位、DNA含量、质膜外表面磷脂酰丝氨酸暴露情况以及细胞活力，分析了类黄酮槲皮素（QU）的抗氧化或促氧化作用。采用多色流式细胞术评估同一细胞中的几个功能参数。在短时间处理时，QU发挥抗氧化作用（H₂O₂水平降低），而长时间处理时则显示促氧化活性（[此处原文缺失具体增加的物质]增加）。在这些条件下，GSH含量降低，这与QU缺乏抗氧化活性相关，而这又可能与该分子的促凋亡活性相关。因此，QU可根据暴露时间和氧化平衡，特别是GSH储备，发挥不同的作用（抗氧化/促氧化）。
