Yu Chuan-ding, Xu Shen-hua, Mou Han-zhou, Jiang Zhi-ming, Zhu Chi-hong, Liu Xiang-lin
Zhejiang Cancer Research Institute, Hangzhou 310022, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2005 Nov;8(6):520-3.
To study the difference of gene expression profiles in gastric cancer (T), pericancerous mucosa (P) and the gastric mucosa from distant cutting margin (C), and to screen an associated novel gene in early gastric carcinogenesis by oligonucleotide microarray.
U133A (Affymetrix, Santa Clara, CA) gene chip was used to detect the gene expression profile difference in T, P and C, respectively. Bioinformatics was used to analyze the detected results.
When gastric cancer was compared with normal gastric mucosa, 766 genes were found,with a difference of more than four times in expression levels, including 530 up-regulated [Signal Log Ratio (SLR) > 2], and 236 down-regulated (SLR< -2). When P was compared with C, 64 genes were found, with a difference of more than four times in expression levels, including 50 up-regulated (SLR > 2), and 14 down-regulated (SLR< -2). Compared with C, a total of 143 genes with a difference of more than four times in expression levels both in T and P tissues. Of the 143 genes, 108 were up-regulated (SLR > 2), and 35 were down-regulated (SLR< -2).
Gene chip can reveal 143 same genes both in pericancerous mucosa and gastric mucosa. These genes may be related to the carcinogenesis and development of early gastric cancer.
研究胃癌组织（T）、癌旁黏膜组织（P）及远切缘胃黏膜组织（C）基因表达谱的差异，采用寡核苷酸芯片筛选早期胃癌发生相关的新基因。
分别应用U133A（Affymetrix，加利福尼亚州圣克拉拉）基因芯片检测T、P、C组织的基因表达谱差异，运用生物信息学方法分析检测结果。
胃癌组织与正常胃黏膜组织比较，共发现766个基因表达水平差异4倍以上，其中上调基因530个[信号对数比值（SLR）>2]，下调基因236个（SLR<-2）。P与C比较，共发现64个基因表达水平差异4倍以上，其中上调基因50个（SLR>2），下调基因14个（SLR<-2）。T与P组织中共有143个基因表达水平差异4倍以上，其中上调基因108个（SLR>2），下调基因35个（SLR<-2）。
基因芯片可揭示癌旁黏膜组织和胃黏膜组织中143个相同的基因，这些基因可能与早期胃癌的发生发展相关。
