浆液性与黏液性卵巢交界性肿瘤中促凋亡蛋白（p53、p21、bax、bak和fas）及抗凋亡蛋白（bcl-2和bcl-x）的表达

Expression of pro-apoptotic (p53, p21, bax, bak and fas) and anti-apoptotic (bcl-2 and bcl-x) proteins in serous versus mucinous borderline ovarian tumours.

作者信息

Fauvet Raffaèle, Dufournet Charlotte, Poncelet Christophe, Uzan Catherine, Hugol Danièle, Daraï Emile

机构信息

Service de Gynécologie-Obstétrique, Hôpital Tenon, AP-HP, CancerEst, Université Pierre et Marie Curie Paris VI, Paris, France.

出版信息

J Surg Oncol. 2005 Dec 15;92(4):337-43. doi: 10.1002/jso.20424.

DOI:10.1002/jso.20424

PMID:16299808

Abstract

BACKGROUND

We examined the expression of apoptosis-related proteins in serous versus mucinous borderline ovarian tumours, in comparison with benign and malignant ovarian tumours.

MATERIALS AND METHODS

Immunohistochemical expression of pro-apoptotic (p53, p21, bax, bak, fas) and anti-apoptotic proteins (bcl-2, bcl-x) was determined in 34 borderline (19 mucinous, 15 serous), 20 benign (10 mucinous, 10 serous) and 28 malignant ovarian tumours (9 mucinous, 19 serous).

RESULTS

A difference in semi-quantitative p53 expression was found between benign and borderline tumours (P = 0.01), but not between borderline and malignant tumours. Increased p21 expression was found in borderline versus benign tumours (P = 0.004). Bcl-2 expression was lower in borderline than in benign (P = 0.01) and malignant tumours (P = 0.02). No difference in bax, bak, fas or bcl-x expression was observed among the three tumour types. Higher percentage of p21 positive cells was found in serous than in mucinous borderline tumours (P < 0.001). Bcl-2 expression was higher in serous than in mucinous forms of benign (P < 0.001), borderline (P < 0.001), and malignant tumours (P < 0.003). No difference in p53, bax, bak, fas or bcl-x expression was observed between serous and mucinous borderline ovarian tumours.

CONCLUSION

Although p53 overexpression was a common feature of both mucinous and serous borderline tumours, p21 and bcl-2 overexpression appeared specific to serous tumours.

摘要

背景

我们研究了浆液性与黏液性卵巢交界性肿瘤中凋亡相关蛋白的表达，并与良性和恶性卵巢肿瘤进行比较。

材料与方法

测定了34例交界性肿瘤（19例黏液性、15例浆液性）、20例良性肿瘤（10例黏液性、10例浆液性）和28例恶性卵巢肿瘤（9例黏液性、19例浆液性）中促凋亡蛋白（p53、p21、bax、bak、fas）和抗凋亡蛋白（bcl-2、bcl-x）的免疫组化表达。

结果

良性肿瘤与交界性肿瘤之间p53的半定量表达存在差异（P = 0.01），但交界性肿瘤与恶性肿瘤之间无差异。交界性肿瘤与良性肿瘤相比，p21表达增加（P = 0.004）。Bcl-2在交界性肿瘤中的表达低于良性肿瘤（P = 0.01）和恶性肿瘤（P = 0.02）。在三种肿瘤类型中，bax、bak、fas或bcl-x的表达无差异。浆液性交界性肿瘤中p21阳性细胞的百分比高于黏液性交界性肿瘤（P < 0.001）。在良性（P < 0.001）、交界性（P < 0.001）和恶性肿瘤（P < 0.003）的浆液性肿瘤中，Bcl-2的表达高于黏液性肿瘤。在浆液性和黏液性卵巢交界性肿瘤之间，p53、bax、bak、fas或bcl-x的表达无差异。