Sommerhalter Monika, Saleh Lana, Bollinger J Martin, Rosenzweig Amy C
Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA.
Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1649-54. doi: 10.1107/S0907444905034062. Epub 2005 Nov 19.
A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6(1)22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal site. The structure of the Mn(II)-loaded form was determined to 2.6 Angstroms resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.
