Laudanski Krzysztof, De Asit, Pellegrini Joanmarie, Miller-Graziano Carol
Department of Surgery, University of Rochester Medical Center, 601 Elmwood Ave.-SURG, Rochester, NY 14642, USA.
Clin Immunol. 2006 Feb-Mar;118(2-3):332-41. doi: 10.1016/j.clim.2005.10.005. Epub 2005 Nov 21.
Possible defective trauma patients' Mphi-T-cell feedback interactions between T cell IL-13 production and IL-1beta and IL-18 Mphi secretion were assessed. Mphi produced IL-1 and IL-18 augment T cell IL-13, which in turn limits excessive macrophage activation. Immunodepressed patients' T cells (depressed proliferation to alphaCD3 + alphaCD4) had decreased IL-13 production concomitant to aberrant Mphi activities ( upward arrow mTNFalpha, downward arrow IL-10) and consequent multiple organ failure (MOF). Decreased IL-13 levels in patients' T cell and diminished Mphi supernatant augmentation of healthy controls' T cell IL-13 production appeared concomitantly, suggesting patients' aberrant monokine levels might intensify in vivo T cell dysfunction severity. Patients' Mphi supernatants, which failed to augment controls' T cell IL-13 production, had depressed IL-1beta and lower induction of IL-18 than immunocompetent patients' Mphi, but combined addition of IL-1beta and IL-18 restored these Mphis' IL-13 enhancing activity. These data suggest that immunodepressed patients' aberrant monokine and depressed T cell IL-13 production are independent but synergistic contributors to emergence of MOF.
评估了创伤患者中可能存在缺陷的巨噬细胞- T细胞反馈相互作用，即T细胞白细胞介素-13产生与白细胞介素-1β和白细胞介素-18巨噬细胞分泌之间的相互作用。巨噬细胞产生的白细胞介素-1和白细胞介素-18可增强T细胞白细胞介素-13的产生，进而限制巨噬细胞的过度活化。免疫抑制患者的T细胞（对αCD3 + αCD4的增殖反应降低）白细胞介素-13产生减少，同时巨噬细胞活性异常（肿瘤坏死因子α升高，白细胞介素-10降低），并导致多器官功能衰竭（MOF）。患者T细胞中白细胞介素-13水平降低，且巨噬细胞上清液对健康对照者T细胞白细胞介素-13产生的增强作用减弱，这两者同时出现，提示患者异常的单核因子水平可能会加重体内T细胞功能障碍的严重程度。患者的巨噬细胞上清液不能增强对照者T细胞白细胞介素-13的产生，其白细胞介素-1β水平降低，白细胞介素-18的诱导水平低于免疫功能正常患者的巨噬细胞，但同时添加白细胞介素-1β和白细胞介素-18可恢复这些巨噬细胞增强白细胞介素-13的活性。这些数据表明，免疫抑制患者异常的单核因子和T细胞白细胞介素-13产生减少是多器官功能衰竭发生的独立但协同的因素。
