Winstead Michelle V, Lucas Karin Killermann, Dennis Edward A
Department of Chemistry and Biochemistry and Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0601, USA.
Prostaglandins Other Lipid Mediat. 2005 Dec;78(1-4):55-66. doi: 10.1016/j.prostaglandins.2005.03.004. Epub 2005 May 31.
Damaging reactive oxygen species are released during episodes of ischemia and reperfusion. Some cellular adaptive responses are triggered to protect the injured organ, while other cascades are triggered which potentiate the damage. In these studies, we demonstrate that rat cardiomyocte H9c2 cells release arachidonic acid in response to hydrogen peroxide. In H9c2 cells, arachidonic acid release is attenuated by methyl arachidonyl fluorophosphonate (MAFP) and pyrrophenone, indicating that a phospholipase A2 Group IV enzyme mediates arachidonic acid mobilization. Moreover, hydrogen peroxide alters the cellular morphology of the H9c2 cells, causing drastic cell shrinkage. Because MAFP and pyrrophenone prevent the morphological alterations caused by hydrogen peroxide, these studies show that phospholipase A2 Group IV activity is likely integrally involved in the damage initiated by hydrogen peroxide.
在缺血和再灌注期间会释放具有损伤性的活性氧。会触发一些细胞适应性反应来保护受损器官，而其他级联反应也会被触发，从而加剧损伤。在这些研究中，我们证明大鼠心肌细胞H9c2细胞会响应过氧化氢而释放花生四烯酸。在H9c2细胞中，甲基花生四烯酰氟磷酸酯（MAFP）和吡洛芬可减弱花生四烯酸的释放，这表明磷脂酶A2 IV组酶介导花生四烯酸的动员。此外，过氧化氢会改变H9c2细胞的细胞形态，导致细胞急剧收缩。由于MAFP和吡洛芬可防止过氧化氢引起的形态改变，这些研究表明磷脂酶A2 IV组活性可能与过氧化氢引发的损伤密切相关。
