Tanikawa M, Acosta T J, Fukui T, Murakami S, Korzekwa A, Skarzynski D J, Piotrowska K K, Park C K, Okuda K
Laboratory of Reproductive Endocrinology, Department of Animal Science, Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan.
Prostaglandins Other Lipid Mediat. 2005 Dec;78(1-4):279-90. doi: 10.1016/j.prostaglandins.2005.09.003. Epub 2005 Oct 27.
Interleukin (IL)-1 has been suggested to participate in regulation of many reproductive functions. To investigate the possible role of IL-1alpha as a local regulator in bovine endometrium, we determined the effects of IL-1alpha on prostaglandin (PG) E2 and PGF(2alpha) output by the bovine endometrium at different stages of the estrous cycle. The expressions of IL-1alpha and IL-1 receptor type 1 (IL-1RT1) mRNA in bovine endometrium were also studied. Bovine uteri were classified into six stages (estrus: day 0; early luteal: days 2-3; developing luteal: days 5-6; mid luteal: days 8-12; late luteal: days 15-17; and follicular: days 19-21). After 1h of pre-incubation, endometrial tissues (20-30mg) were exposed to 0 or 10ng/ml IL-1alpha for 4h. IL-1alpha significantly stimulated PGE2 output throughout the luteal stages, with the highest response during the mid luteal stage, while it did not stimulate PGE2 output during the estrus and the follicular stage. On the other hand, IL-1alpha significantly enhanced PGF(2alpha) output throughout the estrous cycle except in the endometrium from the mid luteal stage, with the highest response at the follicular stage (P<0.001). The treatment of endometrial tissue with IL-1alpha resulted in an increase of the PGE2:PGF(2alpha) ratio at the mid luteal stage, and in a decrease during the late luteal and follicular stages of the estrous cycle. A semiquantitative reverse transcription-polymerase chain reaction revealed that IL-1alpha and IL-1RT1 mRNA are expressed in the endometrium throughout the estrous cycle. IL-1alpha mRNA expression was greater in the early luteal stage than in the estrus, late luteal, and follicular stages (P<0.05). IL-1RT1 mRNA was greater in the late luteal stage than in the other stages (P<0.05). The overall results suggest that IL-1alpha is produced in bovine endometrium throughout the estrous cycle, and plays some roles not only in maintenance of CL, but also in luteolysis by regulating the local PGE2:PGF(2alpha) ratio in bovine endometrium during the estrous cycle.
白细胞介素（IL）-1已被认为参与多种生殖功能的调节。为了研究IL-1α作为牛子宫内膜局部调节因子的可能作用，我们测定了IL-1α对发情周期不同阶段牛子宫内膜前列腺素（PG）E2和PGF（2α）分泌量的影响。还研究了牛子宫内膜中IL-1α和1型IL-1受体（IL-1RT1）mRNA的表达。牛子宫分为六个阶段（发情期：第0天；黄体早期：第2 - 3天；黄体发育中：第5 - 6天；黄体中期：第8 - 12天；黄体晚期：第15 - 17天；卵泡期：第19 - 21天）。预孵育1小时后，将子宫内膜组织（20 - 30毫克）暴露于0或10纳克/毫升的IL-1α中4小时。IL-1α在整个黄体期显著刺激PGE2分泌，在黄体中期反应最强，而在发情期和卵泡期不刺激PGE2分泌。另一方面，IL-1α在整个发情周期中显著增强PGF（2α）分泌，但黄体中期的子宫内膜除外，在卵泡期反应最强（P<0.001）。用IL-1α处理子宫内膜组织导致黄体中期PGE2:PGF（2α）比值升高，而在发情周期的黄体晚期和卵泡期降低。半定量逆转录-聚合酶链反应显示，IL-1α和IL-1RT1 mRNA在整个发情周期的子宫内膜中均有表达。IL-1α mRNA在黄体早期的表达高于发情期、黄体晚期和卵泡期（P<0.05）。IL-1RT1 mRNA在黄体晚期的表达高于其他阶段（P<0.05）。总体结果表明，IL-1α在整个发情周期的牛子宫内膜中产生，不仅在维持黄体方面发挥作用，而且在发情周期中通过调节牛子宫内膜局部PGE2:PGF（2α）比值在黄体溶解中也发挥一定作用。
