Bedir Özlem, Tavares Pereira Miguel, Rehrauer Hubert, Grazul-Bilska Anna, Kowalewski Mariusz P
Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich (UZH), Winterthurerstrasse 260, Zürich, CH-8057, Switzerland.
Department of Histology and Embryology, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey.
BMC Genomics. 2024 Dec 18;25(1):1216. doi: 10.1186/s12864-024-10799-w.
Imbalanced diet and exogenous gonadotrophins affect uterine function and morphology. In sheep, FSH-induced superovulation alters implantation-related gene expression, influenced by both treatment and diet. In this study, we used deep RNA sequencing (NGS, RNA-Seq) to expand our understanding of these effects on the caruncular endometrium.
Ewes (n = 3-5/group) were separated into control fed (CF), overfed (OF), and underfed (UF) groups, with each group subdivided between FSH (superovulated; SOV) or saline (negative controls; CONT) treatment. Caruncular samples were collected on day 10 of diestrus of the subsequent estrous cycle, with samples from CF_CONT also collected on day 5 to assess time-dependent changes.
The 1484 differentially expressed genes (DEGs, P < 0.01, FDR < 0.05) identified between CF_CONT animals at days 5 and 10 were predominantly associated with increased immune activity and cellular metabolic processes and cellular proliferation. In CONT animals, imbalanced nutrition (i.e., both OF and UF) was associated with enrichment of terms associated with cell adhesion and differentiation, immune response and angiogenesis. The FSH carry-over effects resulted in a higher number of DEGs in CF animals (1374), than in OF (168) or UF (18), mostly associated with dysregulation of cell cycle and hormonal sensitivity.
The absence of genes concurrently affected by superovulation (SOV) in all feeding regimes indicates that the effects of FSH on the caruncular transcriptome are multidirectional and dependent upon body condition. Therefore, the homeostasis of ovine caruncles is influenced by both body condition and superovulation (SOV), potentially affecting uterine receptivity.
饮食不均衡和外源性促性腺激素会影响子宫功能和形态。在绵羊中，促卵泡素（FSH）诱导的超排卵会改变与着床相关的基因表达，这受到治疗和饮食的双重影响。在本研究中，我们使用深度RNA测序（NGS，RNA-Seq）来加深对这些因素对肉阜子宫内膜影响的理解。
将母羊（每组n = 3 - 5只）分为对照饲养组（CF）、过度饲养组（OF）和营养不足组（UF），每组再分为FSH（超排卵；SOV）或生理盐水（阴性对照；CONT）处理组。在随后发情周期的间情期第10天收集肉阜样本，同时也收集CF_CONT组第5天的样本以评估时间依赖性变化。
在第5天和第10天的CF_CONT动物之间鉴定出的1484个差异表达基因（DEGs，P < 0.01，FDR < 0.05）主要与免疫活性增加、细胞代谢过程和细胞增殖有关。在CONT动物中，营养不均衡（即OF和UF）与细胞黏附与分化、免疫反应和血管生成相关术语的富集有关。FSH的残留效应导致CF动物中DEGs的数量（1374个）高于OF（168个）或UF（18个），这些DEGs大多与细胞周期失调和激素敏感性有关。
在所有饲养方式下均未发现同时受超排卵（SOV）影响的基因，这表明FSH对肉阜转录组的影响是多方向的，且取决于身体状况。因此，绵羊肉阜的内环境稳定受身体状况和超排卵（SOV）的影响，可能会影响子宫的接受性。
