Leung K-P, Crowe T D, Abercrombie J J, Molina C M, Bradshaw C J, Jensen C L, Luo Q, Thompson G A
Microbiology Branch, US Army Dental and Trauma Research Detachment, Walter Reed Army Institute of Research, 310B, B Street, Building 1H, Great Lakes, IL 60088, USA.
J Dent Res. 2005 Dec;84(12):1172-7. doi: 10.1177/154405910508401215.
Oral biofilms are mixed-species microbial communities, and their uncontrolled outgrowth can express as oral diseases. Antimicrobial peptides represent alternative classes of antimicrobials that exhibit selectivity for prokaryotes. We wanted to test the effect of a synthetic decapeptide antimicrobial, KSL, on the development of oral biofilms formed by isolated human salivary bacteria. We used differential interference contrast microscopy, coupled with a dual-flow cell system, to determine the effect of KSL on oral biofilm development. We used reductions of viable counts and confocal microscopy to assess the bactericidal activity of KSL on mature oral biofilms. KSL effectively blocked biofilm development. A significant effect on the viability of mature biofilms was observed when KSL was used in the presence of a surface-active agent, or after biofilms were mechanically disrupted. This study shows that KSL may be a useful adjunct for conventional oral hygiene to prevent plaque-mediated dental diseases.
口腔生物膜是多种微生物群落，其不受控制的生长会表现为口腔疾病。抗菌肽是一类对原核生物具有选择性的新型抗菌剂。我们想要测试一种合成十肽抗菌剂KSL对分离出的人类唾液细菌形成的口腔生物膜发育的影响。我们使用微分干涉相差显微镜结合双流细胞系统来确定KSL对口腔生物膜发育的影响。我们使用活菌计数减少和共聚焦显微镜来评估KSL对成熟口腔生物膜的杀菌活性。KSL有效地阻止了生物膜的发育。当在表面活性剂存在的情况下使用KSL，或在生物膜受到机械破坏后使用KSL时，观察到对成熟生物膜的活力有显著影响。这项研究表明，KSL可能是传统口腔卫生预防菌斑介导的牙科疾病的有用辅助手段。
