利用肽连接将硒代半胱氨酸掺入蛋白质中。
Incorporation of selenocysteine into proteins using peptide ligation.
作者信息
Hondal Robert J
机构信息
Department of Biochemistry, Vermont College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
出版信息
Protein Pept Lett. 2005 Nov;12(8):757-64. doi: 10.2174/0929866054864319.
Abstract
Expressed protein ligation has become a frequently used technique to insert non-standard amino acids into proteins. The technique has been adapted to insert selenocysteine residues in place of cysteine residue in proteins, taking advantage of the similarity in the chemistries of sulfur and selenium. This replacement can confer unique structural and catalytic properties to enzymes and proteins. The development of this technique also allows for naturally occurring selenoproteins to be produced semisynthetically.
摘要
表达性蛋白质连接已成为一种常用技术，用于将非标准氨基酸插入蛋白质中。利用硫和硒化学性质的相似性，该技术已被用于在蛋白质中插入硒代半胱氨酸残基以取代半胱氨酸残基。这种取代可以赋予酶和蛋白质独特的结构和催化特性。该技术的发展还使得天然存在的硒蛋白能够通过半合成的方式产生。
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