Intein-mediated, in vitro and in vivo protein modifications with small molecules.

作者信息

Tan Lay Pheng, Yao Shao Q

机构信息

Department of Biological Sciences, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore.

出版信息

Protein Pept Lett. 2005 Nov;12(8):769-75. doi: 10.2174/0929866054864193.

DOI:10.2174/0929866054864193

PMID:16305547

Abstract

We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.

摘要