[霍乱弧菌JS94484菌株中CTXEVC Phi和nct-CTXnew Phi基因组中突变基因的序列分析]
[Sequence analysis of the mutated genes in CTXEVC Phi and nct-CTXnew Phi genomes in Vibrio cholerae JS94484 strain].
作者信息
Rui Yong-yu, Kan Biao, Gao Shou-yi, Liu Yan-qing, Qi Guo-ming
机构信息
Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2005 Nov;25(11):1361-4.
OBJECTIVE
To analyze the sequences of the mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genomes in Vibrio cholerae JS94484 strain.
METHODS
The mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genome were obtained by PCR, sequenced and analyzed.
RESULTS
ig1, rstR, ig2, and ctxAB genes in CTX(EVC)Phi genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTX(new)Phi genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTX(EVC)Phi and nct-CTX(new)Phi genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961.
CONCLUSION
ig1, rstR and ig2 genes of nct-CTX(new)Phigenome are of a novel type, and their functions await further investigation.
目的
分析霍乱弧菌JS94484菌株中CTX(EVC)Phi和nct - CTX(new)Phi基因组中突变基因的序列。
方法
通过PCR获得CTX(EVC)Phi和nct - CTX(new)Phi基因组中的突变基因，进行测序和分析。
结果
霍乱弧菌JS94484菌株CTX(EVC)Phi基因组中的ig1、rstR、ig2和ctxAB基因与标准EVC菌株N16961的高度同源，而该菌株nct - CTX(new)Phi基因组中的ig1、rstR和ig2基因与其他3种霍乱弧菌生物型的同源性较低。CTX(EVC)Phi和nct - CTX(new)Phi基因组中zot基因的最后60 bp存在显著差异，表明这两个基因编码的Zot蛋白的氨基酸序列可能存在差异。JS94484菌株的毒素共调节菌毛A亚基基因（tcpA）序列与N16961菌株相同。
结论
nct - CTX(new)Phi基因组的ig1、rstR和ig2基因是新型的，其功能有待进一步研究。
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