Alamsjah Mochammad Amin, Hirao Shotaro, Ishibashi Fumito, Fujita Yuji
Graduate School of Science and Technology, Nagasaki University, Japan.
Biosci Biotechnol Biochem. 2005 Nov;69(11):2186-92. doi: 10.1271/bbb.69.2186.
Thirty-seven species of seaweeds including 10 Chlorophyta, 13 Phaeophyta, and 14 Rhodophyta collected from the coast of Nagasaki Prefecture, Japan, were screened for algicidal activity against the red-tide phytoplankton Heterosigma akashiwo. The green alga Ulva fasciata (Ulvaceae, Chlorophyta) showed the strongest algicidal activity among the seaweeds tested. Bioassay-guided fractionation of the methanol extract of U. fasciata led to isolation of three algicidal compounds whose structures were determined to be hexadeca-4,7,10,13-tetraenoic acid (HDTA), octadeca-6,9,12,15-tetraenoic acid (ODTA), and alpha-linolenic acid on the basis of spectroscopic information. These polyunsaturated fatty acids (PUFAs) showed potent algicidal activity against H. akashiwo (LC(50) 1.35 microg/ml, 0.83 microg/ml, and 1.13 microg/ml for HDTA, ODTA, and alpha-linolenic acid, respectively), and the result demonstrated the potential of these PUFAs for practical harmful algal bloom control.
从日本长崎县海岸采集了37种海藻，包括10种绿藻、13种褐藻和14种红藻，对它们针对赤潮浮游植物赤潮异弯藻的杀藻活性进行了筛选。在所测试的海藻中，绿藻石莼（石莼科，绿藻门）表现出最强的杀藻活性。对石莼甲醇提取物进行生物测定导向的分级分离，得到了三种杀藻化合物，根据光谱信息确定其结构分别为十六碳-4,7,10,13-四烯酸（HDTA）、十八碳-6,9,12,15-四烯酸（ODTA）和α-亚麻酸。这些多不饱和脂肪酸（PUFAs）对赤潮异弯藻表现出强大的杀藻活性（HDTA、ODTA和α-亚麻酸的半数致死浓度分别为1.35微克/毫升、0.83微克/毫升和1.13微克/毫升）；结果表明这些多不饱和脂肪酸在实际控制有害藻华方面具有潜力。
