通过RNA干扰揭示秀丽隐杆线虫中的基因相互作用。

Uncover genetic interactions in Caenorhabditis elegans by RNA interference.

作者信息

Fortunato Angelo, Fraser Andrew G

机构信息

The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Cambridge, Hinxton, UK.

出版信息

Biosci Rep. 2005 Oct-Dec;25(5-6):299-307. doi: 10.1007/s10540-005-2892-7.

DOI:10.1007/s10540-005-2892-7

PMID:16307378

Abstract

RNA-mediated interference (RNAi) has emerged recently as one of the most powerful functional genomics tools. RNAi has been particularly effective in the nematode worm C. elegans where RNAi has been used to analyse the loss-of-function phenotypes of almost all predicted genes. In this review, we illustrate how RNAi has been used to analyse gene function in C. elegans as well as pointing to some future directions for using RNAi to examine genetic interactions in a systematic manner.

摘要

RNA介导的干扰（RNAi）最近已成为最强大的功能基因组学工具之一。RNAi在秀丽隐杆线虫中特别有效，在该线虫中，RNAi已被用于分析几乎所有预测基因的功能丧失表型。在这篇综述中，我们阐述了RNAi如何被用于分析秀丽隐杆线虫中的基因功能，并指出了一些未来利用RNAi系统研究遗传相互作用的方向。