Sivanandan C, Sujatha T P, Prasad Anand Mohan, Resminath R, Thakare Dhiraj R, Bhat S R
National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110012, India.
Biochim Biophys Acta. 2005 Dec 20;1731(3):202-8. doi: 10.1016/j.bbaexp.2005.10.006. Epub 2005 Nov 2.
From a T-DNA tagged Arabidopsis population, a line, M-57 showing GUS (beta-glucuronidase) expression in the vascular regions of young roots was identified. Southern analysis revealed presence of a single T-DNA insert. Using inverse PCR, the plant sequence flanking the T-DNA insertion was cloned. The insertion was identified to be in the intergenic area between loci At4G13940 and At4G13930, coding for SAHH (S-Adenosyl-l-Homocysteine Hydrolase) and SHMT (Serine Hydroxy Methyl Transferase) genes, respectively. A 452-bp fragment immediately upstream of the T-DNA insertion when cloned and mobilized as a GUS fusion was capable of driving a similar root-specific expression of reporter gene in transgenic Arabidopsis plants and their progenies. This cryptic promoter element does not show the presence of any known root-specific promoter element.
从一个T-DNA标签的拟南芥群体中，鉴定出一个名为M-57的株系，该株系在幼根的维管区域显示出GUS（β-葡萄糖醛酸酶）表达。Southern分析显示存在单个T-DNA插入。使用反向PCR，克隆了T-DNA插入侧翼的植物序列。该插入被鉴定位于分别编码SAHH（S-腺苷-L-高半胱氨酸水解酶）和SHMT（丝氨酸羟甲基转移酶）基因的At4G13940和At4G13930位点之间的基因间隔区。当作为GUS融合克隆并转移时，T-DNA插入上游紧邻的一个452 bp片段能够驱动报告基因在转基因拟南芥植物及其后代中产生类似的根特异性表达。这种隐蔽的启动子元件未显示出任何已知的根特异性启动子元件。
