Reimers Mark, Heilig Markus, Sommer Wolfgang H
Laboratory of Molecular Pharmacology, NCI, National Institutes of Health, Bethesda, USA.
Methods. 2005 Nov;37(3):219-28. doi: 10.1016/j.ymeth.2005.09.002.
We describe methods and software tools for doing data analysis based on Affymetrix microarray data, emphasizing often neglected issues. In our experience with neuroscience studies, experimental design and quality assessment are vital. We also describe in detail the pre-processing methods we have found useful for Affymetrix data. Finally, we summarize the statistical literature and describe some pitfalls in the post-processing analysis.
我们描述了基于Affymetrix微阵列数据进行数据分析的方法和软件工具，重点强调了那些常常被忽视的问题。根据我们在神经科学研究中的经验，实验设计和质量评估至关重要。我们还详细描述了我们发现对Affymetrix数据有用的预处理方法。最后，我们总结了统计文献，并描述了后处理分析中的一些陷阱。
