Khurtsiia M Sh, Pavlenishvili I V, Agladze N Dzh, Mitagvariia N P, Beklaia G L
Georgian Med News. 2005 Oct(127):70-3.
Seven days old rats (n=30) were divided into five groups (6 animals in each). Three groups underwent hypoxic-ischemic event (occlusion of right common carotid artery lasting 2,5 hours and breathing with 8% oxygen and 92% of nitrogen mixture). The rats of the fifth group, after completion of hypoxic-ischemic impact and following a daily reoxygenation (for three days) were injected (i/p) with selective inhibitor of nitric oxide inducible synthase, in a dose of 300 mg/kg. The rats of the fourth group were injected with 150 mg/kg of the selective inhibitor of nitric oxide synthase -- aminoguanidine (150 mg/kg); the animals of the third group were injected with saline (50 ml). The second group contained the shame-operated animals and the first group -- intact (control) animals. An intensity of local cerebral blood flow in fronto-parietal area of neocortex was measured by means of hydrogen clearance technique. In the right hemisphere (ipsilateral to occluded common carotid artery) of the animals of the third group significant decrease, and in contralateral hemisphere (left) temporal increase of CBF did occur. Administration of aminoguanidine prevents high degree of brain damage observed in the control group of animals.
将7日龄大鼠（n = 30）分为五组（每组6只动物）。三组经历缺氧缺血事件（右侧颈总动脉闭塞持续2.5小时，并用8%氧气和92%氮气混合物呼吸）。第五组大鼠在缺氧缺血影响完成后，每天进行复氧（持续三天），然后腹腔注射一氧化氮诱导型合酶的选择性抑制剂，剂量为300 mg/kg。第四组大鼠注射150 mg/kg的一氧化氮合酶选择性抑制剂——氨基胍（150 mg/kg）；第三组动物注射生理盐水（50 ml）。第二组为假手术动物，第一组为完整（对照）动物。通过氢清除技术测量新皮质额顶叶区域的局部脑血流强度。在第三组动物的右半球（与闭塞的颈总动脉同侧），脑血流显著减少，而在对侧半球（左）颞叶，脑血流增加。给予氨基胍可防止对照组动物中观察到的高度脑损伤。
