• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA.结合DNA的四聚体内切酶SfiI结构所呈现的连续结合事件。
EMBO J. 2005 Dec 7;24(23):4198-208. doi: 10.1038/sj.emboj.7600880. Epub 2005 Nov 24.
2
A homology model of restriction endonuclease SfiI in complex with DNA.限制性内切酶SfiI与DNA复合物的同源模型。
BMC Struct Biol. 2005 Jan 24;5:2. doi: 10.1186/1472-6807-5-2.
3
Fidelity of DNA sequence recognition by the SfiI restriction endonuclease is determined by communications between its two DNA-binding sites.SfiI限制性内切酶对DNA序列识别的保真度由其两个DNA结合位点之间的通讯决定。
J Mol Biol. 2008 Dec 19;384(3):557-63. doi: 10.1016/j.jmb.2008.09.057. Epub 2008 Oct 2.
4
Communications between catalytic sites in the protein-DNA synapse by the SfiI endonuclease.SfiI核酸内切酶在蛋白质-DNA突触中催化位点之间的通讯。
J Mol Biol. 2002 Apr 26;318(2):387-94. doi: 10.1016/S0022-2836(02)00019-0.
5
A switch in the mechanism of communication between the two DNA-binding sites in the SfiI restriction endonuclease.SfiI限制性内切核酸酶中两个DNA结合位点之间通讯机制的转变。
J Mol Biol. 2007 Nov 9;373(5):1169-83. doi: 10.1016/j.jmb.2007.08.030. Epub 2007 Aug 21.
6
Crystal structure of restriction endonuclease BglI bound to its interrupted DNA recognition sequence.与间断性DNA识别序列相结合的限制性内切酶BglI的晶体结构。
EMBO J. 1998 Sep 15;17(18):5466-76. doi: 10.1093/emboj/17.18.5466.
7
SfiI endonuclease activity is strongly influenced by the non-specific sequence in the middle of its recognition site.SfiI核酸内切酶的活性受到其识别位点中间非特异性序列的强烈影响。
Nucleic Acids Res. 2001 Apr 1;29(7):1476-83. doi: 10.1093/nar/29.7.1476.
8
DNA looping by the Sfi I restriction endonuclease.Sfi I限制性内切酶介导的DNA环化
J Mol Biol. 1998 Aug 21;281(3):433-44. doi: 10.1006/jmbi.1998.1967.
9
Alternative geometries of DNA looping: an analysis using the SfiI endonuclease.DNA 环化的替代几何结构：使用 SfiI 核酸内切酶的分析
J Mol Biol. 2000 May 5;298(3):461-75. doi: 10.1006/jmbi.2000.3676.
10
Crystallization of restriction endonuclease SfiI in complex with DNA.限制性内切酶SfiI与DNA复合物的结晶。
Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1493-5. doi: 10.1107/s0907444903011910. Epub 2003 Jul 23.

引用本文的文献

1
Burkholderia cenocepacia epigenetic regulator M.BceJIV simultaneously engages two DNA recognition sequences for methylation.中慢生假单胞菌表观遗传调节因子 M.BceJIV 同时与两个 DNA 识别序列结合进行甲基化。
Nat Commun. 2024 Sep 7;15(1):7839. doi: 10.1038/s41467-024-52130-x.
2
epigenetic regulator M.BceJIV simultaneously engages two DNA recognition sequences for methylation.表观遗传调控因子M.BceJIV同时结合两个DNA识别序列进行甲基化。
bioRxiv. 2024 Jan 23:2024.01.20.576384. doi: 10.1101/2024.01.20.576384.
3
Assembly of Synaptic Protein-DNA Complexes: Critical Role of Non-Specific Interactions.突触蛋白-DNA 复合物的组装：非特异性相互作用的关键作用。
Int J Mol Sci. 2023 Jun 6;24(12):9800. doi: 10.3390/ijms24129800.
4
Site-Search Process for Synaptic Protein-DNA Complexes.突触蛋白-DNA复合物的位点搜索过程
Int J Mol Sci. 2021 Dec 25;23(1):212. doi: 10.3390/ijms23010212.
5
Structural basis for human ZBTB7A action at the fetal globin promoter.人类 ZBTB7A 在胎儿珠蛋白启动子上作用的结构基础。
Cell Rep. 2021 Sep 28;36(13):109759. doi: 10.1016/j.celrep.2021.109759.
6
Structure of HhaI endonuclease with cognate DNA at an atomic resolution of 1.0 Å.以 1.0Å 的原子分辨率解析 HhaI 内切酶与其同源 DNA 的结构。
Nucleic Acids Res. 2020 Feb 20;48(3):1466-1478. doi: 10.1093/nar/gkz1195.
7
The cell cycle-regulated DNA adenine methyltransferase CcrM opens a bubble at its DNA recognition site.细胞周期调控的 DNA 腺嘌呤甲基转移酶 CcrM 在其 DNA 识别位点打开一个泡。
Nat Commun. 2019 Oct 10;10(1):4600. doi: 10.1038/s41467-019-12498-7.
8
DNA Conformation Induces Adaptable Binding by Tandem Zinc Finger Proteins.DNA 构象诱导串联锌指蛋白的适应性结合。
Cell. 2018 Mar 22;173(1):221-233.e12. doi: 10.1016/j.cell.2018.02.058. Epub 2018 Mar 15.
9
Role for first zinc finger of WT1 in DNA sequence specificity: Denys-Drash syndrome-associated WT1 mutant in ZF1 enhances affinity for a subset of WT1 binding sites.WT1 第一个锌指在 DNA 序列特异性中的作用：与 Denys-Drash 综合征相关的 WT1 突变体在 ZF1 中增强了对一组 WT1 结合位点的亲和力。
Nucleic Acids Res. 2018 May 4;46(8):3864-3877. doi: 10.1093/nar/gkx1274.
10
Structural basis of human PR/SET domain 9 (PRDM9) allele C-specific recognition of its cognate DNA sequence.人类PR/SET结构域9（PRDM9）等位基因C对其同源DNA序列的特异性识别的结构基础。
J Biol Chem. 2017 Sep 29;292(39):15994-16002. doi: 10.1074/jbc.M117.805754. Epub 2017 Aug 11.

本文引用的文献

1
Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence.基于与BglII DNA序列结合的BstYI结构对切换限制酶特异性的启示。
Structure. 2005 May;13(5):791-801. doi: 10.1016/j.str.2005.02.018.
2
Structure of HinP1I endonuclease reveals a striking similarity to the monomeric restriction enzyme MspI.HinP1I核酸内切酶的结构显示出与单体限制性酶MspI惊人的相似性。
Nucleic Acids Res. 2005 Apr 1;33(6):1892-901. doi: 10.1093/nar/gki337. Print 2005.
3
Type II restriction endonucleases: structure and mechanism.II型限制性核酸内切酶：结构与机制
Cell Mol Life Sci. 2005 Mar;62(6):685-707. doi: 10.1007/s00018-004-4513-1.
4
A homology model of restriction endonuclease SfiI in complex with DNA.限制性内切酶SfiI与DNA复合物的同源模型。
BMC Struct Biol. 2005 Jan 24;5:2. doi: 10.1186/1472-6807-5-2.
5
REBASE--restriction enzymes and DNA methyltransferases.REBASE——限制性内切酶和DNA甲基转移酶。
Nucleic Acids Res. 2005 Jan 1;33(Database issue):D230-2. doi: 10.1093/nar/gki029.
6
An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site.限制性内切酶MspI在其回文DNA识别位点上的不对称复合物。
Structure. 2004 Sep;12(9):1741-7. doi: 10.1016/j.str.2004.07.014.
7
Dynamics of DNA loop capture by the SfiI restriction endonuclease on supercoiled and relaxed DNA.SfiI限制性内切核酸酶对超螺旋和松弛DNA的DNA环捕获动力学
J Mol Biol. 2004 May 21;339(1):53-66. doi: 10.1016/j.jmb.2004.03.046.
8
Crystal structure of BstYI at 1.85A resolution: a thermophilic restriction endonuclease with overlapping specificities to BamHI and BglII.分辨率为1.85埃的嗜热限制性内切酶BstYI的晶体结构：对BamHI和BglII具有重叠特异性的嗜热限制性内切酶。
J Mol Biol. 2004 May 7;338(4):725-33. doi: 10.1016/j.jmb.2004.02.074.
9
3DNA: a software package for the analysis, rebuilding and visualization of three-dimensional nucleic acid structures.3DNA：一个用于三维核酸结构分析、重建和可视化的软件包。
Nucleic Acids Res. 2003 Sep 1;31(17):5108-21. doi: 10.1093/nar/gkg680.
10
Crystallization of restriction endonuclease SfiI in complex with DNA.限制性内切酶SfiI与DNA复合物的结晶。
Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1493-5. doi: 10.1107/s0907444903011910. Epub 2003 Jul 23.

结合DNA的四聚体内切酶SfiI结构所呈现的连续结合事件。

A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA.

作者信息

Vanamee Eva Scheuring, Viadiu Hector, Kucera Rebecca, Dorner Lydia, Picone Stephen, Schildkraut Ira, Aggarwal Aneel K

机构信息

Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, NY 10029, USA.

出版信息

EMBO J. 2005 Dec 7;24(23):4198-208. doi: 10.1038/sj.emboj.7600880. Epub 2005 Nov 24.

DOI:10.1038/sj.emboj.7600880
PMID:16308566
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1356319/
Abstract

Many reactions in cells proceed via the sequestration of two DNA molecules in a synaptic complex. SfiI is a member of a growing family of restriction enzymes that can bind and cleave two DNA sites simultaneously. We present here the structures of tetrameric SfiI in complex with cognate DNA. The structures reveal two different binding states of SfiI: one with both DNA-binding sites fully occupied and the other with fully and partially occupied sites. These two states provide details on how SfiI recognizes and cleaves its target DNA sites, and gives insight into sequential binding events. The SfiI recognition sequence (GGCCNNNN[downward arrow]NGGCC) is a subset of the recognition sequence of BglI (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer and BglI is a dimer, and there is little sequence similarity between the two enzymes, their modes of DNA recognition are unusually similar.

摘要

细胞中的许多反应是通过在突触复合物中隔离两个DNA分子来进行的。SfiI是一个不断增加的限制酶家族的成员，它可以同时结合并切割两个DNA位点。我们在此展示了与同源DNA结合的四聚体SfiI的结构。这些结构揭示了SfiI的两种不同结合状态：一种是两个DNA结合位点都被完全占据，另一种是位点被完全和部分占据。这两种状态提供了关于SfiI如何识别和切割其靶DNA位点的详细信息，并深入了解了连续的结合事件。SfiI识别序列（GGCCNNNN[向下箭头]NGGCC）是BglI识别序列（GCCNNNN[向下箭头]NGGC）的一个子集，并且这两种酶都切割它们的靶DNA以留下3个碱基的3'突出端。我们表明，尽管SfiI是四聚体而BglI是二聚体，并且这两种酶之间几乎没有序列相似性，但它们的DNA识别模式异常相似。