人类结直肠癌中一组共调节因子和三种核受体的表达分析

Expression analysis of a subset of coregulators and three nuclear receptors in human colorectal carcinoma.

作者信息

Giannini Romina, Cavallini Aldo

机构信息

Laboratory of Biochemistry, National Institute for Digestive Diseases, I.R.C.C.S. Saverio de Bellis, 70013-Castellana Grotte, BA, Italy.

出版信息

Anticancer Res. 2005 Nov-Dec;25(6B):4287-92.

PMID:16309230

Abstract

In human colorectal tissue samples, the gene expressions of 4 coactivators, p300, pCAF, TIF-2 and TRAP 220, and 7 corepressors, N-CoR, REA, MTA1, MTA1L1, HDAC1, HDAC2 and HDAC3, linked to estrogen receptors (ER), were revealed by traditional RT-PCR. Cofactors ERalpha, ERbeta and ERRalpha mRNA levels were then measured in 40 tumor tissue samples matched with respective normal mucosa by real-time PCR. The decline of mRNA levels of all coactivators and the increase of NCoR, HDAC1, HDAC2 and MTA1 were observed from normal to tumor tissue, whereas REA, HDAC3 and MTA1L1 expressions were similar in both tissue compartments. The gene expression of ERbeta correlated with those of p300, TIF-2 and REA in normal mucosa, and with that of REA in tumor tissue only. No association was found between ERalpha and coregulators and between each coregulator and different clinical parameters. Our findings suggest that the co-induction of ERbeta and some cofactors may play an important role during the development of human colorectal carcinoma.

摘要

在人类结肠组织样本中，通过传统逆转录聚合酶链反应（RT-PCR）揭示了与雌激素受体（ER）相关的4种共激活因子（p300、pCAF、TIF-2和TRAP 220）以及7种共抑制因子（N-CoR、REA、MTA1、MTA1L1、HDAC1、HDAC2和HDAC3）的基因表达。然后，通过实时PCR测定了40例肿瘤组织样本及其相应正常黏膜中辅因子ERα、ERβ和ERRα的mRNA水平。从正常组织到肿瘤组织，观察到所有共激活因子的mRNA水平下降，以及NCoR、HDAC1、HDAC2和MTA1的增加，而REA、HDAC3和MTA1L1在两个组织区域中的表达相似。在正常黏膜中，ERβ的基因表达与p300、TIF-2和REA的基因表达相关，而仅在肿瘤组织中与REA的基因表达相关。未发现ERα与共调节因子之间以及各共调节因子与不同临床参数之间存在关联。我们的研究结果表明，ERβ和一些辅因子的共同诱导可能在人类结肠癌的发生发展过程中起重要作用。