来自膜叶嘉赐树的细胞毒性克罗烷二萜类化合物。

Cytotoxic clerodane diterpenoids from Casearia membranacea.

作者信息

Shen Ya-Ching, Cheng Yuan-Bin, Ahmed Atallah F, Lee Cheng Lin, Chen Shun-Ying, Chien Ching-Te, Kuo Yao-Haur, Tzeng Guo-Lin

机构信息

Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan, Republic of China.

出版信息

J Nat Prod. 2005 Nov;68(11):1665-8. doi: 10.1021/np058063o.

DOI:10.1021/np058063o

PMID:16309320

Abstract

Bioassay-guided fractionation of the EtOAc extract of Casearia membranacea leaves and twigs afforded three new clerodane diterpenes, caseamembrins M-O (1-3), and the known rel-(2S,5R,6R,8S,9S,10R,18S,19R)-2-(2-methylbutyryloxy)-6-hydroxy-18,19-di-O-acetyl-18,19-epoxycleroda-3,13(16),14-triene (4) and caseamembrin D (5). The structures of 1-3, including the relative configurations, were established by extensive NMR spectroscopic analyses. The cytotoxic activities of the isolated diterpenoids against human oral epidermoid (KB), medulla (Med), and colon (DLD-1) cancer cell lines were evaluated.

摘要

对膜叶嘉赐树叶和嫩枝的乙酸乙酯提取物进行生物测定导向的分级分离，得到了三种新的克罗烷二萜类化合物，即膜叶嘉赐素M - O（1 - 3），以及已知的rel -（2S，5R，6R，8S，9S，10R，18S，19R）- 2 -（2 - 甲基丁酰氧基）- 6 - 羟基 - 18,19 - 二 - O - 乙酰基 - 18,19 - 环氧克罗达 - 3,13(16),14 - 三烯（4）和膜叶嘉赐素D（5）。通过广泛的核磁共振光谱分析确定了1 - 3的结构，包括相对构型。评估了分离得到的二萜类化合物对人口腔表皮样（KB）、髓质（Med）和结肠（DLD - 1）癌细胞系的细胞毒性活性。