Zhang Li, Li Wen-Hwa, Anthonavage Michael, Eisinger Magdalena
The Johnson and Johnson Skin Research Center, CPPW, 199 Grandview Road, Skillman, NJ 08558, USA.
Peptides. 2006 Feb;27(2):413-20. doi: 10.1016/j.peptides.2005.05.030. Epub 2005 Nov 22.
Melanocortin receptors (MC1R-MC5R) and their ligands (melanocyte-stimulating hormone (MSH) and adrenocorticotrophin hormone (ACTH)) have been shown to influence physiological functions of cells and organs, including exocrine glands. Since relatively little is known about MC5R expression and function in the human sebaceous gland, we examined expression of MC5R by immunohistochemistry and RT-PCR in human sebaceous cells in vivo and in vitro. In human skin, MC5R was detected only in differentiating, lipid-laden sebaceous cells but not in basal, undifferentiated sebaceous cells. Similarly, in cultured human sebocytes MC5R was only detectable at the onset of differentiation and in fully differentiated cells displaying prominent lipid granules. The lipid profile of the cultured and differentiated human sebocytes was shown to be human sebum-specific using (14)C-acetate labeling and high performance thin layer chromatography. Our studies suggest that MC5R is a marker of human sebocyte differentiation.
黑皮质素受体（MC1R - MC5R）及其配体（促黑素细胞激素（MSH）和促肾上腺皮质激素（ACTH））已被证明会影响细胞和器官的生理功能，包括外分泌腺。由于关于MC5R在人皮脂腺中的表达和功能了解相对较少，我们通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测了MC5R在体内和体外人皮脂腺细胞中的表达。在人皮肤中，仅在正在分化、充满脂质的皮脂腺细胞中检测到MC5R，而在基底未分化的皮脂腺细胞中未检测到。同样，在培养的人皮脂腺细胞中，仅在分化开始时以及在显示出明显脂质颗粒的完全分化细胞中可检测到MC5R。使用（14）C - 醋酸盐标记和高效薄层色谱法表明，培养和分化的人皮脂腺细胞的脂质谱具有人皮脂特异性。我们的研究表明，MC5R是人皮脂腺细胞分化的标志物。
