Stanimirovic Zoran, Stevanovic Jevrosima, Jovanovic Slobodan, Andjelkovic Marko
Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. oslobodjenja 18, Belgrade 11000, Serbia and Montenegro.
Mutat Res. 2005 Dec 30;588(2):152-7. doi: 10.1016/j.mrgentox.2005.10.003. Epub 2005 Nov 23.
Apitol, with cymiazole hydrochloride as the active ingredient, is used in bee-keeping against the ectoparasitic mite Varroa destructor. The preparation was evaluated for genotoxicity in cultured human peripheral blood lymphocytes. Sister chromatid exchange, the mitotic index and the cell proliferation index were determined for three experimental concentrations of Apitol (0.001, 0.01 and 0.1 mg/ml). All concentrations significantly (p < 0.001) increased the mitotic index (MI = 7.35+/-0.18%, 8.31+/-0.20% and 12.33+/-0.25%, respectively), the proliferative index (PI = 1.83+/-0.01, 1.84+/-0.01 and 1.88+/-0.02, respectively) and the frequency of sister chromatid exchange (SCE = 8.19+/-1.81, 8.78+/-1.80 and 13.46+/-1.88, respectively), suggesting that cymiazole hydrochloride has genotoxic potential.
以盐酸嘧菌唑为活性成分的Apitol用于养蜂业防治外寄生螨狄斯瓦螨。对该制剂在培养的人外周血淋巴细胞中的遗传毒性进行了评估。测定了Apitol三个实验浓度（0.001、0.01和0.1毫克/毫升）下的姐妹染色单体交换、有丝分裂指数和细胞增殖指数。所有浓度均显著（p < 0.001）提高了有丝分裂指数（分别为MI = 7.35±0.18%、8.31±0.20%和12.33±0.25%）、增殖指数（分别为PI = 1.83±0.01、1.84±0.01和1.88±0.02）以及姐妹染色单体交换频率（分别为SCE = 8.19±1.81、8.78±1.80和13.46±1.88），表明盐酸嘧菌唑具有遗传毒性潜力。
