Rong Jun, Song Zhiping, Su Jun, Xia Hui, Lu Bao-Rong, Wang Feng
The Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, China.
New Phytol. 2005 Dec;168(3):559-66. doi: 10.1111/j.1469-8137.2005.01539.x.
Crop-to-crop transgene flow will affect seed purity of non-GM rice varieties, leading to unwanted consequences. To assess the maximum probability of transgene outflow in rice (Oryza sativa), gene flow experiments were conducted with three cultivation patterns with different mixed-planting proportions of adjacent GM and non-GM rice at two sites in Fujian and Hainan Provinces of China. Three GM rice lines containing two insect-resistance genes (Bt/CpTI) and their non-GM counterparts were used in the experiments to allow natural hybridization to occur. A hygromycin resistance gene was used as a selective marker for identifying hybrids. Based on the examination of > 645 700 geminated seeds, the result showed low frequencies (0.05-0.79%) of transgene flow from GM to non-GM rice at close spacing, although with significant variation among mixed-planting proportions. It is concluded that rice transgene flow will occur at a very low frequency (< 1.0%), even if the GM rice is planted at close spacing with non-GM rice, and high densities of GM rice cultivated in the neighborhood of non-GM rice will increase the probability of outcrossing with the non-GM rice.
作物间的转基因漂移会影响非转基因水稻品种的种子纯度，从而导致不良后果。为评估水稻（Oryza sativa）中转基因外流的最大可能性，在中国福建省和海南省的两个地点，采用三种种植模式，设置转基因水稻与非转基因水稻不同的邻作混种比例，开展了基因漂移实验。实验使用了三个含有两种抗虫基因（Bt/CpTI）的转基因水稻品系及其对应的非转基因品系，以使自然杂交发生。使用潮霉素抗性基因作为鉴定杂种的选择标记。通过对超过645700粒发芽种子的检测，结果表明，近距离种植时，转基因从转基因水稻流向非转基因水稻的频率较低（0.05 - 0.79%），尽管在混种比例间存在显著差异。得出的结论是，即使转基因水稻与非转基因水稻近距离种植，水稻转基因漂移的频率也会非常低（<1.0%），且在非转基因水稻附近种植高密度转基因水稻会增加与非转基因水稻异交的概率。
