Dezulian Tobias, Remmert Michael, Palatnik Javier F, Weigel Detlef, Huson Daniel H
Center for Bioinformatics Tübingen, Tübingen University, Germany.
Bioinformatics. 2006 Feb 1;22(3):359-60. doi: 10.1093/bioinformatics/bti802. Epub 2005 Nov 29.
MicroRNAs (miRNAs) are a recently discovered class of non-coding RNAs that regulate gene and protein expression in plants and animals. MiRNAs have so far been identified mostly by specific cloning of small RNA molecules, complemented by computational methods. We present a computational identification approach that is able to identify candidate miRNA homologs in any set of sequences, given a query miRNA. The approach is based on a sequence similarity search step followed by a set of structural filters.
微小RNA(miRNAs)是最近发现的一类非编码RNA,可调节动植物中的基因和蛋白质表达。到目前为止,miRNAs大多是通过小RNA分子的特异性克隆来鉴定的,并辅以计算方法。我们提出了一种计算鉴定方法,给定一个查询miRNA,该方法能够在任何序列集中鉴定候选miRNA同源物。该方法基于序列相似性搜索步骤,随后是一组结构筛选。