Gogoleva E V, Grechushkina N N, Egorov N S
Mikrobiologiia. 1975 Sep-Oct;44(5):828-31.
Mycobacterium lacticolum 121 grows and synthesizes exopolysaccharide at the initial values of pH of the medium from 5.0 to 11.5. The accumulation of biomass is maximal (10 to 12 gram/litre) at pH 6.0 to 11.5; the rate of growth is highest at pH 8.0 to 11.5. The largest amount of exopolysaccharide (2.0 to 2.4 gram/litre) is produced in the medium at pH 8.0 to 11.0; the rate of its production is highest at pH 9.0 to 11.0. The growth of Mycobacterium lacticolum 121 and the biosynthesis of polysaccharide are optimal at pH 8.0 to 8.2. Changes in acidity of the medium have no effect on the qualitative composition, structure, and molecular weight of the polysaccharide.
乳酸分支杆菌121在培养基初始pH值为5.0至11.5时生长并合成胞外多糖。在pH 6.0至11.5时生物量积累最大(10至12克/升);在pH 8.0至11.5时生长速率最高。在pH 8.0至11.0的培养基中产生的胞外多糖量最大(2.0至2.4克/升);在pH 9.0至11.0时其产生速率最高。乳酸分支杆菌121的生长和多糖的生物合成在pH 8.0至8.2时最为适宜。培养基酸度的变化对多糖的定性组成、结构和分子量没有影响。