Dong Wei-Jiang, Hu Hai-Tao, Gong Hui-Li
Department of Anatomy and Histology, Medical College of Xi'an Jiaotong University, Xi'an, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Oct;30(5):553-7.
To investigate the tumor-suppression effect of PA combined with GM-CSF, TNF-alpha and IL-4 on cord blood mononuclear cells (CBMC).
The mononuclear cells were isolated from human umbilical cord blood and cultured with polyacttin A (PA), GM-CSF + TNF-alpha + IL-4 (GTI), and GTI + PA (GTIP) respectively. Six days later, surface antigen expression of the cultured cells, including CD1a and CD83, which were the specialized markers of dendritic cell (DC), were analyzed by immunohistochemistry technique. The CBMC were cultured with GTI for 24 h to enhance DC, then were added apoptotic/necrotic Hela/HepG2 tumor cells, and finally PA was co-cultured. The antitumor cytotoxicity of CBMC was measured by MTT assay.
After the culture, CD1a and CD83 positive cell rates of the PA group inreased significantly, reaching (19.63 +/- 3.61)%, (9.28 +/- 4.31) % respectively, much higher than that of the control, but lower than that of the GTI group. The killing rate to the tumor cells of CBMC cultured with GTIP increased remarkably, much higher than the control, GTI and PA groups. After tumor antigens were added to the CBMC of GTIP group (GTIP + Tc), the killing rate increased.
PA not only promotes the proliferation and maturation of cord blood derived DC, but also improves the tumor-suppression effect of CBMC cultured with GTI.
探讨多抗肌动蛋白A（PA）联合粒细胞巨噬细胞集落刺激因子（GM-CSF）、肿瘤坏死因子-α（TNF-α）和白细胞介素-4（IL-4）对脐血单个核细胞（CBMC）的抑瘤作用。
从人脐带血中分离单个核细胞，分别用多抗肌动蛋白A（PA）、GM-CSF+TNF-α+IL-4（GTI）以及GTI+PA（GTIP）进行培养。6天后，采用免疫组织化学技术分析培养细胞的表面抗原表达，包括作为树突状细胞（DC）特异性标志物的CD1a和CD83。将CBMC用GTI培养24小时以增强DC，然后加入凋亡/坏死的Hela/HepG2肿瘤细胞，最后与PA共同培养。通过MTT法测定CBMC的抗肿瘤细胞毒性。
培养后，PA组的CD1a和CD83阳性细胞率显著增加，分别达到（19.63±3.61）%、（9.28±4.31）%，远高于对照组，但低于GTI组。用GTIP培养的CBMC对肿瘤细胞的杀伤率显著提高，远高于对照组、GTI组和PA组。在GTIP组的CBMC中加入肿瘤抗原（GTIP+Tc）后，杀伤率增加。
PA不仅促进脐血来源DC的增殖和成熟，还提高了用GTI培养的CBMC的抑瘤效果。
