Zhang Hui-Qiong, Yi Zhu-Wen, He Xiao-Jie, Dang Xi-Qiang, He Qing-Nan, Mo Shuang-Hong
Laboratory of Pediatric Nephrology, Second Xiangya Hospital, Central South University, Changsha, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Oct;30(5):597-600.
To investigate the difference of Pax2 and P53 expressions in children with primary nephritic syndrome (PNS) and the effect of Pax2 on glucocorsteroid (GC)-resistance.
Renal Pax2 and P53 expressions in children with PNS (40 patients) were detected by immunohistochemistry. A semiquantitative score was used to evaluate the injury degree of the glomeruli and the tubulointerstitium, and correlation analysis was done among Pax2, P53 and pathologic score.
Pax2 and P53 expressions were not found in the control group. Pax2 expression of renal tubule epithelia exsisted in children with PNS and there was weak or no expression of Pax2 in the podocytes. Pax2 expressions in the proximal tubule and the distal tubule in the GC-resistant group were more intense than those in the GC-intensive group (P <0.01). The more the Pax2 expression in the tubule, the more abnormal structure such as dilation and atrophy. Pax2 expression in the tubule epithelia was positively correlated with pathologic score of tubulointerstitium (P < 0.01). There was no P53 expression in the GC-intensive group, but there exsisted P53 expression in parts of the patients from the GC-resistant group, mainly distributing in the renal tubular epithelia. P53 expression was positively correlated with P53 expression and the pathologic score of tubulointerstitium (P < 0.01).
Over-expression of Pax2 in the renal tubule epithelia may improve P53 expression to a certain degree, which may aggravate the lesion of the renal tubule. It may be one of the mechanisms resulting in GC-resistant in children with PNS.
探讨原发性肾病综合征（PNS）患儿中Pax2和P53表达的差异以及Pax2对糖皮质激素（GC）抵抗的影响。
采用免疫组织化学法检测40例PNS患儿肾组织中Pax2和P53的表达。用半定量评分法评估肾小球和肾小管间质的损伤程度，并对Pax2、P53与病理评分进行相关性分析。
对照组未检测到Pax2和P53表达。PNS患儿肾小管上皮细胞有Pax2表达，足细胞中Pax2表达较弱或无表达。GC抵抗组近端小管和远端小管中Pax2表达较GC敏感组更强烈（P<0.01）。肾小管中Pax2表达越多，扩张、萎缩等结构异常越明显。肾小管上皮细胞中Pax2表达与肾小管间质病理评分呈正相关（P<0.01）。GC敏感组无P53表达，而GC抵抗组部分患儿存在P53表达，主要分布于肾小管上皮细胞。P53表达与Pax2表达及肾小管间质病理评分呈正相关（P<0.01）。
肾小管上皮细胞中Pax2过表达可能在一定程度上上调P53表达，进而加重肾小管病变，这可能是PNS患儿GC抵抗的机制之一。
