Chang Wook, Um Youngsoon, Pulliam Holoman Tracey R
Department of Chemical Engineering, University of Maryland, College Park, 20742, USA.
Biotechnol Prog. 2005 Nov-Dec;21(6):1789-94. doi: 10.1021/bp050250p.
Anaerobic benzene degradation was confirmed in microbial communities enriched from Baltimore Harbor (Baltimore, MD) sediments under methanogenic conditions. Molecular characterization based on 16S rDNA gene sequences revealed that the strains in the communities were diversely affiliated with such phylogenetic branches as the Bacteroidetes, Euryarchaeota, Firmicutes, and Thermotogae phyla. Of interest was that the majority of the microbial populations detected in these cultures were closely related to the members of dechlorinating microbial communities. Further, some of those species were previously found in naphthalene- or phenanthrene-degrading methanogenic communities. Finally, this result could be used to design targeted isolation strategies for anaerobic benzene-degrading strains under methanogenic conditions.
在产甲烷条件下，从巴尔的摩港（马里兰州巴尔的摩）沉积物中富集的微生物群落中证实了厌氧苯降解。基于16S rDNA基因序列的分子表征表明，群落中的菌株与拟杆菌门、广古菌门、厚壁菌门和栖热袍菌门等系统发育分支有不同程度的关联。有趣的是，在这些培养物中检测到的大多数微生物种群与脱氯微生物群落的成员密切相关。此外，其中一些物种先前在降解萘或菲的产甲烷群落中被发现。最后，这一结果可用于设计在产甲烷条件下厌氧苯降解菌株的靶向分离策略。
