对局部应用蛋白质的抗原反应。
Antigenic response to topically applied proteins.
作者信息
Harrison E F, Fuquay M E, Zygmunt W A
出版信息
Infect Immun. 1975 Feb;11(2):309-12. doi: 10.1128/iai.11.2.309-312.1975.
Six different proteins varying widely in molecular weight, ribonuclease, lysostaphin, ovalbumin, penicillinase, collagenase, and Varidase were tested for their ability to induce circulating antibody formation in rabbits after repeated topical application of the proteins in a water-soluble gel vehicle. After a 12-week exposure period, significant hemagglutinin titers were noted in rabbits treated with ovalbumin, lysostaphin, or ribonuclease; markedly elevated, passive cutaneous anaphylaxis-reacting sera were obtained only from collagenase- or lysostaphin-treated animals. Precipitin antibodies as evidenced by gel diffusion were also found in sera from collagenas- and lysostaphin-treated animals. Topical application of penicillinase was only marginally effective and Varidase was totally ineffective in elicting a positive circulating antibody response. In all cases, topical application of proteins for periods in excess of 3 weeks was required for induction of circulating antibody formation.
对六种分子量差异很大的不同蛋白质（核糖核酸酶、溶葡萄球菌酶、卵清蛋白、青霉素酶、胶原酶和Varidase）进行了测试，在以水溶性凝胶载体反复局部应用这些蛋白质后，观察它们诱导兔体内循环抗体形成的能力。经过12周的暴露期后，在用卵清蛋白、溶葡萄球菌酶或核糖核酸酶处理的兔中观察到显著的血凝素滴度；仅从用胶原酶或溶葡萄球菌酶处理的动物中获得了明显升高的被动皮肤过敏反应血清。在经胶原酶和溶葡萄球菌酶处理的动物血清中也发现了凝胶扩散所证实的沉淀素抗体。局部应用青霉素酶的效果微乎其微，而Varidase在引发阳性循环抗体反应方面则完全无效。在所有情况下，诱导循环抗体形成需要局部应用蛋白质超过3周的时间。
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